Innovations in Flow Cytometry & Extracellular Vesicles 2024
Date: Monday, 18 November 2024 - Wednesday, 20 November 2024
Location: The Hills Hotel -- Laguna Hills, California
Confirmed Speakers
Alison Fujii, Field Application Scientist, ONI Inc.
Eric Diebold
WW Vice President, Research and Development, BD Biosciences
Jimmy Fay, Field Application Scientist, NanoFCM
Joshua Welsh, Staff Scientist, Advanced Technology Group, Becton Dickinson
Michael Graner
Professor, University of Colorado Anschutz School of Medicine -- Conference Co-Chair
Sven Rudolf Kreutel, Chief Executive Officer, Particle Metrix GmbH and CEO, Particle Metrix Inc., USA
Daniel Chiu
A. Bruce Montgomery Professor of Chemistry, University of Washington
Gregory Cooksey, Project Leader, National Institute of Standards and Technology (NIST)
John Nolan, CEO, Cellarcus Biosciences, Inc.
Lynn Pulliam, Professor of Laboratory Medicine and Medicine, University of California-San Francisco
Ramin Hakami, Co-Director of the Center for Infectious Disease Research, George Mason University
Terry Morgan, Professor, Oregon Health & Science University (OHSU)
Dino Di Carlo
Armond and Elena Hairapetian Chair in Engineering and Medicine, Professor and Chair of Bioengineering, University of California Los Angeles -- Chairperson
Jean-Luc Fraikin, CEO, Spectradyne
Joseph de Rutte, CEO and Co-Founder, Partillion Bioscience
Malgorzata Witek, Associate Research Professor, University of Kansas
Steve Soper
Foundation Distinguished Professor, Director, Center of BioModular Multi-scale System for Precision Medicine, The University of Kansas
Yu-Hwa Lo
Professor, University of California San Diego
Innovations in Flow Cytometry & Extracellular Vesicles 2024
Welcome to the SelectBIO Innovations in Flow Cytometry and Extracellular Vesicles 2024 Conference to be held in-person on-site in beautiful Laguna Hills, California.
Bringing together researchers and industry participants from both academia and industry, this established event, will discuss the latest innovations and developments in EV/Exosomes fields from a Technology Development and Utilization Perspective.
A heavy emphasis at this conference on technology development especially in the context of flow cytometers for a variety of emerging applications -- the engagement of academic researchers and industry participants is a key element of this conference track.
There is an Extensive International Perspective at this Conference with Speakers, Poster Presenters, Sponsors, and Exhibitors from the US, Europe, and Asia/Pacific. Running alongside the conference will be an exhibition covering the latest technological advances and associated products and services from leading solution providers within this expanding field from around the world.
Registered delegates will have full access to the co-located and concurrent tracks and sessions to mix-and-match presentations and maximize networking:
• Lab-on-a-Chip and Microfluidics: Techologies, Companies and Commercialization 2024
• Innovations in Flow Cytometry and Extracellular Vesicles (EVs) 2024
• Organoids, Spheroids and Organs-on-Chips 2024
There is an extensive exhibit hall featuring companies from these fields enabling business development, partnering and networking. Extensive networking opportunities for partnering, collaborations and business development available over the course of this 2.5-day in-person conference with onsite breakfasts, lunches, beer and wine and dinner receptions.
Call for Papers and Posters
Call for Papers
If you would like to be considered for an oral presentation at this meeting, Submit an abstract for review now!
Oral Presentation Submission Deadline: 31 March 2024
Call for Posters
You can also present your research on a poster while attending the meeting. Submit an abstract for consideration now!
Poster Submission Deadline: 04 November 2024
Agenda Topics
- • Demos of Flow Cytometry Instrumentation by the Technology Developers
• Development in Flow Cytometry: Technologies and Applications
• Instrumentation
• Reagents and Applications
• Downstream Analysis of Biomarker Cargo Contained in Extracellular Vesicles (EVs)/Exosomes
• Microfluidics Tools for Single Cell Analysis
• Single Cell and Vesicle Studies by Flow Cytometry and Microfluidics
• Tools & Technologies for Isolation of Extracellular Vesicles (EVs)/Exosomes
Sponsorship and Exhibition Opportunities
Jeff Fan
Exhibition Manager - SelectBIO
E-mail: Jeff@selectbioconferences.com
3 for 2 Offer on Delegate Registrations
SelectBIO are offering 3 Delegate Registrations for the price of 2 on all delegate passes. To take advantage of this offer, please contact us by email, phone or click the Contact Us button below. Looking for more than 3 Delegate Passes? Contact us for more information on our special rates for large groups.
Any questions or assistance during registration, please call us at: +1 (510) 857-4865 or e-mail us at: Contact SelectBIO
Gold Sponsors
Exhibitors
If you require any information about exhibiting or sponsoring at one of our events please contact Jeff Fan using the information below:
Jeff Fan
Exhibition Manager - SelectBIO
Email: Jeff@selectbioconferences.com
Why exhibit at a SelectBIO Conference?
Specialists: SelectBIO doesn't organize conferences in shipping, accountancy, textiles etc. – just drug discovery and the life sciences. Many of our staff have bioscience qualifications and many years of experience. So, we speak your language and understand your needs.
Superior Customer Service: Our sales team will take care of you with specialist advice and customized packages. We don’t forget you after you sign on the bottom line either as our customer service dept. will alert you to all the things you need to think about up to and during the event itself.
Innovations in Flow Cytometry & Extracellular Vesicles 2024 Venue
SelectBIO is delighted to host the Innovations in Flow Cytometry and Extracellular Vesicles 2024 Conference at The Hills Hotel in Laguna Hills, California.
The Hills Hotel
25205 La Paz Road
Laguna Hills, California 92653, USA
This hotel in Southern California is easily accessible from Los Angeles and San Diego via Interstate-5.
The nearest airport is: John Wayne Airport (SNA) - 13.7 miles from the hotel.
From Los Angeles International Airport (LAX) - the hotel is 52.9 miles.
The hotel is within a short drive of Disneyland and Legoland California, as well as Laguna Beach, Newport Beach, as well as shopping and dining at South Coast Plaza in Costa Mesa.
All conference sessions, exhibition as well as networking reception will be held in the Conference Center at The Hills Hotel.
SelectBIO has negotiated discounted hotel room pricing for conference attendees at The Hills Hotel.
To make your Hotel Reservations Online:
Click the Button Below to Open the Hotel Booking Website
This will provide a SelectBIO discounted rate for booking hotel at the conference venue hotel
For any hotel reservation-related issues, or if you need any help with hotel bookings, please contact:
Jeff Fan
Events Manager, SelectBIO
E-mail: Jeff@selectbioconferences.com
SelectBIO has NOT authorized ANY third party company to assist in hotel bookings or reservations for the conference. Please do NOT do business with any third party companies. If in doubt, please contact Jeff Fan immediately to clarify.
Register to this conference and also enjoy the following co-located events at no extra charge.
Training Courses
If you would like to submit a proposal for an oral or poster presentation at this meeting, please fill out the form below required for your submission.
Successful applicants will be provided with all necessary information.
Abstract Content:
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Written in English
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Written in the third person
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Include title, name(s) and affiliation(s) of the authors
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Between 100 - 200 words
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Suitable for direct publication in the proceedings pack and on the website
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Related to the subject of the conference
Agenda Topics
- • Demos of Flow Cytometry Instrumentation by the Technology Developers
• Development in Flow Cytometry: Technologies and Applications
• Instrumentation
• Reagents and Applications
• Downstream Analysis of Biomarker Cargo Contained in Extracellular Vesicles (EVs)/Exosomes
• Microfluidics Tools for Single Cell Analysis
• Single Cell and Vesicle Studies by Flow Cytometry and Microfluidics
• Tools & Technologies for Isolation of Extracellular Vesicles (EVs)/Exosomes
Copyrights
The presenting author/person who submitted the abstract assumes full responsibility of the content of the abstract and we assume that all co-authors are aware of this content. Please note that your biography, summary and abstract may be used on this website and conference materials.
Alison Fujii Biographical Sketch
Alison Fujii, Field Application Scientist, ONI Inc.
Alison Fujii is a Field Application Scientist at Oxford Nanoimaging, Inc. (ONI). She specializes in extracellular vesicle research using super-resolution microscopy on the ONI Nanoimager. Alison has worked with flow cytometry in cancer immunology research and collaborates with academic labs and bio/pharma industries to advance microscopy techniques for exploring cellular processes at the nanoscale.
Daniel Chiu Biographical Sketch
Daniel Chiu, A. Bruce Montgomery Professor of Chemistry, University of Washington
Daniel T. Chiu is currently the A. Bruce Montgomery Professor Chemistry, Endowed Professor of Analytical Chemistry, and Professor of Bioengineering at the University of Washington. He is a member of the University of Washington’s Center for Nanotechnology, Neurobiology and Behavior Program, and the Cancer Consortium of the Fred Hutchinson Cancer Research Center. He has authored more than 180 publications and is the inventor on over 40 issued patents. Dr. Chiu obtained a B.A. in neurobiology and a B.S. in chemistry at the University of California, Berkeley in 1993, and a Ph.D. in chemistry from Stanford University in 1998.
Dino Di Carlo Biographical Sketch
Dino Di Carlo, Armond and Elena Hairapetian Chair in Engineering and Medicine, Professor and Chair of Bioengineering, University of California-Los Angeles
Dino Di Carlo received his B.S. in Bioengineering from the University of California, Berkeley in 2002 and received a Ph.D. in Bioengineering from the University of California, Berkeley and San Francisco in 2006. From 2006-2008 he conducted postdoctoral studies in the Center for Engineering in Medicine at Harvard Medical School. He has been on the faculty in the Department of Bioengineering at UCLA since 2008 and now as Professor of Bioengineering and Mechanical Engineering serves as the Chair of the Department and as the director of the Cancer Nanotechnology Program in the Jonsson Comprehensive Cancer Center. His research pioneered the use of inertial fluid dynamic effects for the control, separation, and analysis of cells in microfluidic devices. His recent work extends into numerous other fields of biomedicine and biotechnology including directed evolution, cell analysis for rapid diagnostics, new amplified molecular assays, next generation biomaterials, and phenotypic drug screening. He has also been a leader in technology entrepreneurship: He co-founded and currently serves on the board of directors of five companies that are commercializing UCLA intellectual property developed in his lab (CytoVale, Vortex Biosciences, Tempo Therapeutics, Forcyte Biotechnologies and Ferrologix). Among other honors he received the Presidential Early Career Award for Scientists and Engineers (PECASE) and was elected a Fellow of the American Institute for Medical and Biological Engineering in 2016, was elected a Fellow of the Royal Society of Chemistry (FRSC) in 2014, was awarded the National Science Foundation (NSF) Faculty Early Career Development award and the U.S. Office of Naval Research (ONR) Young Investigator Award, the Packard Fellowship and Defense Advanced Research Projects Agency (DARPA) Young Faculty Award, and received the National Institutes of Health (NIH) Director’s New Innovator Award and Coulter Translational Research Award.
Eric Diebold Biographical Sketch
Eric Diebold
WW Vice President, Research and Development, BD Biosciences
Eric Diebold is the Worldwide Vice President of Research and Development for BD Biosciences, where he leads all aspects of product and technology development for the Biosciences business unit within Becton Dickinson and Co., one of the world’s largest medical technology companies. Eric has been with BD Biosciences for the past 7 years, where he has served in roles of increasing responsibility within the R&D function. Prior to BD, Eric was the CEO and Founder of Omega Biosystems Incorporated, which was acquired by BD in 2017. Eric received his BS in Electrical Engineering and Physics from Duke University, and his PhD in Applied Physics from Harvard University. He lives in Menlo Park, CA with his wife and three sons.
Gregory Cooksey Biographical Sketch
Gregory Cooksey, Project Leader, National Institute of Standards and Technology (NIST)
Dr. Cooksey is a Project Leader in the Microsystems and Nanotechnology Division at the National Institute of Standards and Technology (NIST). His lab develops microfluidic platforms with integrated optical structures to measure fluids, particles, and cells with high precision. His team’s optofluidic flow meter is the most accurate flow metrology platform in the world, and it has a response time on the order of 0.1 s at 1 nL/min. Dr. Cooksey also leads a microfluidic cytometry project that uses multiple interrogation regions to extract first ever per-object uncertainty quantification and measurement of dynamics in flow. This technology was awarded a Gold Medal and the 2023 Ron Brown Excellence in Innovation Award by the U.S. Department of Commerce.
Jean-Luc Fraikin Biographical Sketch
Jean-Luc Fraikin, CEO, Spectradyne
Dr Fraikin developed Spectradyne’s initial Microfluidic Resistive Pulse Sensing technology as part of his PhD thesis work at UC Santa Barbara in the research group of Dr. Andrew Cleland. After graduating he received postdoctoral training in cancer cell biology and biochemistry and worked in industry developing microfluidics-based molecular diagnostics. Dr. Fraikin is a co-founder of Spectradyne and now serves as the company’s CEO.
Jimmy Fay Biographical Sketch
Jimmy Fay, Field Application Scientist , NanoFCM
Jimmy received his PhD from the University of North Carolina in the department of Biochemistry and Biophysics. His thesis work focused on the association of protein and polymers in the context of polyion complex nanoparticles. Subsequently, he moved to industry working on implementing a suite of biophysical assays for the quantitative characterization of extracellular vesicles. Currently, he is a Field Application Scientist at NanoFCM where he works to spread awareness of Nano-flow cytometry and implement novel methodologies using the NanoFCM NanoAnalyzer. In particular, his recent focus has been on surface quantification of biological markers and targeting moieties on nanoparticles.
John Nolan Biographical Sketch
John Nolan, CEO, Cellarcus Biosciences, Inc.
Past President of ISAC. Inventor of Cellarcus’ vesicle analysis technologies. Internationally recognized expert in cytometry and vesicle analysis.
Joseph de Rutte Biographical Sketch
Joseph de Rutte, CEO and Co-Founder, Partillion Bioscience
Dr. Joe de Rutte is the Co-Founder and CEO of Partillion Bioscience, a venture-backed startup that is commercializing the Nanovial technology he pioneered during his PhD at UCLA. This technology utilizes hydrogel microparticles to enable researchers to perform microfluidic assays without the need for new, complex instruments. The impact of his work has been recognized by leaders in the field, earning him the top Innovation Award at SLAS 2020 and the New Product Award in 2022. Since its inception, Dr. de Rutte has led Partillion, successfully raising venture capital funding and launching its first commercial products. These developments address a growing need for functional analysis of cells, showcasing the significant contributions of Dr. de Rutte and his team to the scientific community.
Joshua Welsh Biographical Sketch
Joshua Welsh, Staff Scientist, Advanced Technology Group, Becton Dickinson
Joshua Welsh, Ph.D., recently joined BD’s Advanced Technology Group as a Staff Scientist where he works on next-generation standardization. He earned his BSc (Hons) in Physiology with Pharmacology from the University of Leicester and completed his Ph.D. in Translational Medicine at the University of Southampton. Dr. Welsh continued his training at the National Institutes of Health, where he advanced to a Staff Scientist role in Dr. Jennifer Jones' Translational Nanobiology Section. Dr. Welsh led initiatives like MISEV2023 and MIFlowCyt-EV, served as a member-at-large on the board of ISEV, chaired the ISEV reference materials task force, and led the ISEV-ISAC-ISTH EV flow cytometry working group. He is also the developer of FCMPASS, an optimization, calibration, and reporting tool for small particle flow cytometry.
Lynn Pulliam Biographical Sketch
Lynn Pulliam, Professor of Laboratory Medicine and Medicine, University of California-San Francisco
Dr Pulliam received her BA degree from Northwestern University, her MS in Microbiology at California State University at Long Beach and her PhD in Experimental Pathology at the University of California, San Francisco (UCSF). She is a Professor of Laboratory Medicine and Medicine at UCSF and Director of Microbiology at the San Francisco VA Health Care Center. She was the Director of Research at the SF VA, the largest VA research center in the US, for 7 years. She is a founding member and past President of the International Society for Neurovirology (ISNV) as well as the Women in Neuroscience awardee and recipient of the ISNV Pioneer Award. She serves on the Board of Directors of ISNV and the Editorial Boards of several journals. She has also received awards from AmFar, the Northern California Institute for Research and Education and Drexel University Prize in Neurovirology.
Malgorzata Witek Biographical Sketch
Malgorzata Witek, Associate Research Professor, University of Kansas
Dr. Malgorzata (Maggie) A. Witek is an Associate Research Professor at KU, Lawrence. Her research is focused on microfluidic-based isolation and solid-phase extractions of liquid biopsy markers, including circulating tumor cells, extracellular vesicles, and cell free DNA for stroke and cancer disease management. She shares her research efforts between the University of Kansas in the NIH/NIBIB funded Center of BioModular Multiscale Systems for Precision Medicine and the COBRE funded Kansas Institute for Precision Medicine at the University of Kansas Medical Center (KUMC). Maggie co-directs the Liquid Biopsy Core at KUMC campus.
Michael Graner Biographical Sketch
Michael Graner, Professor, Dept of Neurosurgery, University of Colorado Anschutz School of Medicine
Michael Graner received his PhD in Biochemistry from the University of Illinois followed by post-doctoral and research faculty work at the University of Arizona, shifting gears from the Drosophila extracellular matrix to cancer immunotherapy. He then took at faculty position at Duke University’s Tisch Brain Tumor Center, followed by his current position as Professor in Neurosurgery at the University of Colorado Denver (Anschutz Medical Campus). He is also a member of the University of Colorado Cancer Center, the Colorado Clinical and Translational Sciences Institute, the MAVRC Program, and holds a Visiting Professorship Appointment at the Shenzhen Third People’s Hospital (China) and an adjunct faculty appointment at Colorado State University. Graner has a long-standing interest in cell stress responses, which led to cancer vaccine development (including one in clinical trials), which somehow led to the world of extracellular vesicles (EVs). His lab currently concentrates on signaling mechanisms involving EVs, in particular the transfer of stressed phenotypes from stressed tumor cells to unstressed ones via EVs.
Paul Patrone Biographical Sketch
Paul Patrone, Physicist and Staff Scientist, Applied and Computational Mathematics Division, National Institute of Standards and Technology (NIST)
Paul Patrone is a physicist in the Applied and Computational Mathematics Division at NIST with a background in mathematical modeling, stochastics, uncertainty quantification, and analysis. For over a decade he has worked on problems at the interface of applied mathematics, physics, and measurement science. He is a co-inventor on multiple patents and patents-pending, including for the world’s most sensitive flow-meter and a novel microfluidic serial cytometer.
Ramin Hakami Biographical Sketch
Ramin Hakami, Co-Director of the Center for Infectious Disease Research, George Mason University
Dr. Hakami obtained his Ph.D. in Biochemistry in the laboratory of the Nobel Laureate Professor Har Gobind Khorana at the Massachusetts Institute of Technology (MIT), and was subsequently awarded a NRSA fellowship from NIH to complete postdoctoral training at Harvard Medical School (HMS). He is currently the co-director of the Center for Infectious Disease Research at George Mason University. The main focus of research in Dr. Hakami’s laboratory is to understand the fundamental mechanisms by which vesicular trafficking within the host regulates innate immune responses during infection with pathogenic agents. In particular, a major focus is the molecular mechanisms by which extracellular vesicles (EVs) regulate innate immunity. The broad goal of these studies is identifying new strategies for development of highly effective host-based countermeasures.
Steve Soper Biographical Sketch
Steve Soper, Foundation Distinguished Professor, Director, Center of BioModular Multi-scale System for Precision Medicine, The University of Kansas
Prof. Soper is a Foundation Distinguished Professor in Chemistry, Bioengineering, and Mechanical Engineering at the University of Kansas. At KUMC, Prof. Soper holds an adjunct appointment in the Cancer Biology Department and is a member of the KU Cancer Center.
Prof. Soper has secured extramural funding totaling >$135M, has published over 245 peer-reviewed manuscripts (h index = 70; >18,000 citations); 31 book chapters and 91 peer-reviewed conference proceeding papers, and is the author of 20 patents. He is also the founder of a startup company, BioFluidica, which is marketing devices for the isolation and enumeration of liquid biopsy markers. Soper recently founded a second company, Sunflower Genomics, which is seeking to market a new DNA/RNA single-molecule sequencing platform. His list of awards includes Ralph Adams Award in Bioanalytical Chemistry, Chemical Instrumentation by the American Chemical Society, the Benedetti-Pichler Award for Microchemistry, Fellow of the AAAS, Fellow of Applied Spectroscopy, Fellow of the Royal Society of Chemistry, R&D 100 Award, Distinguished Masters Award at LSU and Outstanding Scientist/Engineer in the state of Louisiana in 2001. Finally, Prof. Soper has granted 60 PhDs and 7 MS degrees to students under his mentorship. He currently heads a group of 20 researchers.
Sven Kreutel Biographical Sketch
Sven Kreutel, Particle Metrix GmbH and CEO, Particle Metrix Inc.
Sven Kreutel studied biology at the University of Hohenheim in Stuttgart, Germany. After his graduation in biology, he received his PhD in microbiology and protein biochemistry at the University of Hohenheim under Prof. Dr. Andreas Kuhn and Dr. Dorothee Kiefer working on the light sensing and signal transduction in photosynthetic bacteria. Since then he worked in different sales and sales manager positions within the life science industry from clinical research over biology to nanoparticle sciences. In 2021 he was appointed as CEO of the American subsidiary of Particle Metrix.
Terry Morgan Biographical Sketch
Terry Morgan, Professor, Oregon Health & Science University (OHSU)
Dr. Morgan is a Professor of Pathology and Biomedical Engineering at Oregon Health & Science University (OHSU) in Portland, Oregon, USA. He has been an NIH-funded investigator since 2012 and he has worked with BD Biosciences since 2016 to develop, validate, and begin research trials using nanoscale high resolution flow cytometry to image, count, and isolate extracellular vesicles (EVs). Dr. Morgan is the leader of the EV research group at the Center for Developmental Health at OHSU. He is also the leader of the flow cytometry EV group at the Knight Cancer Institute to test its potential as a diagnostic platform for early cancer detection.
Yu-Hwa Lo Biographical Sketch
Yu-Hwa Lo, Professor, University of California San Diego
Yuhwa Lo received his PhD in electrical engineering from UC Berkeley. He has been a professor of University of California, San Diego since 1999. He is also the director of the UCSD Nano3 (Nanoscience, Nanoengineering, Nanomedicine) Facility. His research interests include microfluidics, lab on a chip, biosensors, biomedical instrument, biophotonics, nanophotonic and optoelectronic devices. He has around 400 publications and been award 31 patents. He is a fellow of the IEEE and the Optical Society of America.
09:00
18 November 2024
Slate Room
Steve Soper, Foundation Distinguished Professor; Director, Center of BioModular Multi-scale System for Precision Medicine, Adjunct Professor, Ulsan National Institute of Science & Technology, The University of Kansas, United States of America
Pre-Conference Training Course from 09:00-11:00
Microfluidics and Nanofluidics for Diagnostic Tests
[Separate Registration Required to Attend this Pre-Conference Training Course]
11:00
18 November 2024
Slate Room
Leanna Levine, Founder & CEO, ALine, Inc. United States of America
Pre-Conference Training Course from 11:00-13:00
Microfluidic Product Development
[Separate Registration Required to Attend this Pre-Conference Training Course]
This training course will explore the translation of science and nascent engineering programs into a well-structured product development program to address Reduction to Practice or proof of concept; Human factors engineering – what is it and why it matters; Design Control and Risk Control Roadmap for Development; Design for Manufacture and Assembly; Scale -up progression and manufacturing methods.
**A Must-Attend for Companies Embarking on Microfluidic Product Development**
13:00
18 November 2024
Conference Entrance
Main Conference Registration, Materials Pick-Up and Networking in the Exhibit Hall
13:30
18 November 2024
Plenary Ballroom
Lab-on-a-Chip and Microfluidics World Congress 2024
Opening Plenary Session
13:45
18 November 2024
Plenary Ballroom
Welcome and Introduction to the Lab-on-a-Chip and Microfluidics World Congress 2024 by the Chairs: Professor Dino Di Carlo and Dr. Leanna Levine
2024 Conference Focus and Themes Highlighted Over the 3-Day Event
14:00
18 November 2024
Plenary Ballroom
View Agenda of Conference Plenary Session on the Agenda Page of the Lab-on-a-Chip and Microfluidics 2024 Track
18:45
18 November 2024
Exhibit Hall
Networking Reception in the Exhibit Hall with Beer, Wine and Dinner.
Network with Colleagues, Engage with the Exhibitors and View Posters.
20:15
18 November 2024
Exhibit Hall
Close of Day 1 of the Conference
20:30
18 November 2024
Slate Room
Noah Malmstadt, Professor of Chemical Engineering and Materials Science, University of Southern California, United States of America
3D-Printing of Microfluidics Training Course
[Separate Registration Required to Attend this Training Course]
07:30
19 November 2024
Exhibit Hall
Morning Coffee, Continental Breakfast and Networking in the Exhibit Hall
08:45
19 November 2024
Ballroom A
For Programming Details for Morning Sessions -- Please see Agenda in Lab-on-a-Chip Track and Organoids Track
12:30
19 November 2024
Exhibit Hall
Networking Buffet Luncheon -- Network with Exhibitors and Colleagues, View Posters
13:20
19 November 2024
Ballroom B
Michael Graner, Professor, Dept of Neurosurgery, University of Colorado Anschutz School of Medicine -- Chairperson
Session Title: Single Cell Analysis and EV Research via Flow Cytometry
13:30
19 November 2024
Ballroom B
Daniel Chiu, A. Bruce Montgomery Professor of Chemistry, University of Washington, United States of America
High-Resolution Analysis of Single Extracellular Vesicles and Particles with Digital Flow Cytometry
We have developed a multi-parametric high-throughput flow-based method for the analysis of individual extracelluar vesicles and particles (EVPs), and a super-resolution method for sizing individual EVPs in a high-throughput fashion. EVPs are highly heterogeneous and comprise a diverse set of surface protein markers as well as intra-vesicular cargoes. Yet, current approaches to the study of EVPs lack the necessary sensitivity and precision to fully characterize and understand the make-up and the distribution of various EV subpopulations that may be present. Digital flow cytometry (dFC) provides single-fluorophore sensitivity and enables multiparameter characterization of EVPs, including single-EVP phenotyping, the absolute quantitation of EVP concentrations, and biomarker copy numbers. dFC has a broad range of applications, from analysis of single EVPs such as exosomes or RNA-binding proteins to characterization of therapeutic lipid nanoparticles, viruses, and proteins. dFC also provides absolute quantitation of non-EVP samples such as dyes, beads, and Ab-dye conjugates.
14:00
19 November 2024
Ballroom B
Yu-Hwa Lo, Professor, University of California San Diego, United States of America
AI Enabled 2D and 3D Image-Guided Cell Analyzers and Sorters
Single cell analysis is playing an important role in biological and medical research. Flow cytometers and fluorescence-activated cell sorters (FACS) are workhorse for single cell analysis, offering high throughput and the ability of isolating cells of high interest, especially those rare cells or new cell types. On the other hand, microscopy, especially 3D microscopy, can produce high information contents, revealing important insight in cell phenotypes and high spatial information although cell isolation in a microscope platform with techniques such as laser microdissection is cumbersome and subject to cell damage. We present results of image-guided cell sorters that possess the merits of high throughput and high content imaging in a single system. The image-guided FACS system uses a microfluidic cartridge with an on-chip piezoelectric actuator to sort cells of interest into tubes or 384-well plates. The cell sorting criteria is based on the 2D images of cells, defined by either operator specified gating criteria or AI with or without human instructions. Going beyond the systems with 2D images, we further demonstrate flow cytometers that produce 3D fluorescent, scattering, and transmission images of each single cell at a throughput of 1000 cells/s, which is 1000-10,000 times faster than a fluorescent confocal microscope. The cell tomography offers far more information than 2D images and is more adaptive to AI and machine learning. Both the 2D and 3D image-guided cell analyzers and sorters are powered by artificial intelligence with convolutional neural networks. With AI capabilities based on semi-supervised learning, both systems have shown exceptional capabilities for classification and cell type discovery. Results from several experiments, including the diagnosis of leukemia and NASH diseases, DNA damage assessment, protein translocations under drug treatments, cell cycle detection, and cell fate prediction (the so-called Day Zero experiment) will be discussed in the presentation.
14:30
19 November 2024
Ballroom B
Herve Tiriac, Scientist, University of California-San Diego
Chemoresistance Drives Cell Intrinsic and Extrinsic Programs in Pancreas Cancer
Pancreas cancer is a deadly disease with a lack of effective treatment options and rapid onset of resistance. Here we use patient-derived organoids and animal models to study therapeutic responses and study mechanisms of resistance.
15:00
19 November 2024
Ballroom B
Joshua Welsh, Staff Scientist, Advanced Technology Group, Becton Dickinson, United States of America
Navigating Small Particle Flow Cytometry
Small particle analysis using flow cytometry has rapidly developed in the last decade and is arguably the most developed flow cytometry discipline with regard to standardization. Consideration and resources for tackling small particle flow cytometry can be daunting for newcomers to the area. This talk will outline key considerations and available resources for making the most out of your samples.
15:30
19 November 2024
Ballroom B
Gregory Cooksey, Project Leader, National Institute of Standards and Technology (NIST), United States of America
A Microfluidic Serial Cytometer to Estimate Per-Cell Uncertainty and Single Object Kinetic Measurements
NIST has developed a serial microfluidic cytometer that repeats the interrogation of objects at multiple points along a flow path, which enables direct assessment of uncertainty in cytometry measurements (DiSalvo et al., Lab Chip 2022). We have achieved per-particle measurement variation below 2 % at throughputs above 100 s-1 and detection limits and dynamic range comparable to conventional cytometers. This presentation will introduce various measurement capabilities novel to our serial cytometry, including 1) uncertainty quantification, 2) signals analysis that permit estimation of velocity, size, and shape of samples, including elastically deformable particles and mitotic cells, and 3) tracking dynamics of individual objects over time. Utilizing microdroplets, we demonstrated temporal tracking of an enzymatic reaction on a per-droplet basis in real time. An upstream fluidic mixing system is also used to create known concentrations and dilutions of fluorophores in droplets, which permits direct calibration of measured fluorescence intensity to fluorophores in solution.
16:00
19 November 2024
Ballroom B
Joseph de Rutte, CEO and Co-Founder, Partillion Bioscience, United States of America
Nanovials: Bridging Microfluidics with Flow Cytometry to Enable Functional Screening of Cells
In this talk, I will provide background on utilizing hydrogel microparticles in conjunction with flow cytometers to screen and isolate individual cells based on their functional properties, such as secretion. I will also expand on how this capability is being leveraged to accelerate antibody discovery workflows by screening antibody secreting cells directly based on antibody function (e.g. specificity, affinity, cell binding).
16:30
19 November 2024
Ballroom B
Ramin Hakami, Co-Director of the Center for Infectious Disease Research, George Mason University, United States of America
Lab-on-a-Chip Platform for Functional Studies of Extracellular Particles
Extracellular particles (EPs) such as small extracellular vesicles (sEV), microvesicles, exomeres, etc., are potent bio-functional nanoparticles released by cells that facilitate intercellular communication. They are constitutively released in vivo and in vitro and can trigger a wide range of biological responses upon uptake by recipient cells. Despite their recognized significance, there are still significant gaps in the understanding of specific functional roles of EPs from different tissue and cell types due to various factors, including the heterogeneity and complexity of EPs. In vitro approaches to investigate EPs typically require costly and/or lengthy purification steps to obtain target EPs of interest. Most importantly, in vitro investigation of EP function commonly involves treating 2D-cultured recipient cells with a certain number of EPs simultaneously, poorly replicating the constant and gradual release of EPs in vivo. To help address these issues, we have developed a microfluidic chip system that facilitates the live exchange of distinct subpopulations of EPs between co-cultured cell populations. The inter-cell region acts as a selective barrier filled with one of several different hydrogels that can permit diffusion of specific size ranges of EPs. Recently, we have also successfully produced second generation of our chip platform with significantly enhanced features. Our optimizations for the chip’s design and fabrication have enhanced production, convenience, and efficiency of use. In addition, they allow the use of a distinct hydrogel formulation to enable exchange of exomere-sized nanoparticles while preventing sEV exchange.
17:00
19 November 2024
Ballroom B
Sven Kreutel, CEO, Particle Metrix, Inc., United States of America
Characterization of Extracellular Vesicles and Other Biological Nanoparticles using Nanoparticle Tracking Analysis (NTA)
Nanoparticle Tracking Analysis (NTA) has emerged as a fast and vital characterization technology for Extracellular Vesicles (EVs), Exosomes and other biological material in the size range from 30 nm to 1 μm. While classic NTA scatter operation feeds back the size and total particle concentration, the user typically cannot discriminate whether the particle is a vesicle, protein aggregate, cellular trash or an inorganic precipitate. The fluorescence detection capabilities of f-NTA however enables the user to gain specific biochemical information for phenotyping of all kinds of vesicles and viruses. Alignment-free switching between excitation wavelengths and measurement modes (scatter and fluorescence) allow quantification of biomarker ratios such as the tetraspanins (CD63, CD81 and CD9) within minutes. Furthermore, specific co-localization studies using c-NTA gives a deeper understanding of the composition of biomarker on single particle.
17:30
19 November 2024
Ballroom B
Alison Fujii, Field Application Scientist, ONI Inc., United States of America
Characterization of Extracellular Vesicles Using the ONI Nanoimager Super-Resolution Microscope
This presentation will explore the characterization of extracellular vesicles (EVs) using the ONI Nanoimager's super-resolution capabilities. Key topics include the importance of EVs in biological research, the advanced imaging techniques of the ONI Nanoimager, and practical applications for detailed EV analysis. Attendees will learn how super-resolution microscopy can provide deeper insights into EV biology.
18:00
19 November 2024
Ballroom B
Jean-Luc Fraikin, CEO, Spectradyne, United States of America
Quantifying Extracellular Vesicles (EVs) in Complex Biofluids with Spectradyne’s ARC(tm) Particle Analyzer
Spectradyne’s ARC particle analyzer measures EV size, concentration & phenotype down to 50 nm diameter. In this presentation, we will demonstrate the ARC’s ability to accurately quantify EV subpopulations directly in serum, without SEC or other significant sample prep. The ARC particle analyzer is uniquely well suited to analyzing complex, heterogeneous samples. Fast and easy to use—no calibration, alignment, or cleaning required—the ARC is rapidly being adopted in EV applications for its accurate and direct measurements of particle size, concentration, and phenotype at the nanoscale.
18:30
19 November 2024
Exhibit Hall
Networking Reception with Beer, Wine and Dinner in the Exhibit Hall -- Network with Exhibitors, Colleagues and View Posters
20:15
19 November 2024
Exhibit Hall
Close of Day 2 Main Conference Programming
20:30
19 November 2024
Slate Room
Shuichi Takayama, Professor, Georgia Research Alliance Eminent Scholar, and Price Gilbert, Jr. Chair in Regenerative Engineering and Medicine, Georgia Institute of Technology & Emory University School of Medicine, United States of America
Introduction to Microfluidics Training Course
[Separate Registration Required to Attend this Training Course]
07:30
20 November 2024
Exhibit Hall
Morning Coffee, Continental Breakfast and Networking in the Exhibit Hall
08:00
20 November 2024
Ballroom A
Industry Breakout Round-Tables: Each Round-Table Moderated by an Industry Participant -- Delegates Engage with the Moderator to Discuss Commercialization Themes
Moderators are:
David Weitz, Harvard – Microfluidics
Roger Kamm, MIT – Organs-on-Chips
Greg Cooksey, NIST – Flow Cytometry
09:00
20 November 2024
Ballroom A and B
For Morning Sessions Talks, See Agenda in LOAC Track and Organoids Track and this Track
09:59
20 November 2024
Ballroom B
Session Title: Exploring Further the Use of Flow Cytometry in the EV Space and Biological Investigations into EVs
10:00
20 November 2024
Ballroom B
Paul Patrone, Physicist and Staff Scientist, Applied and Computational Mathematics Division, National Institute of Standards and Technology (NIST), United States of America
Foundations of Metrology and Uncertainty Quantification in Cytometry
Uncertainty quantification (UQ) for cytometry remains a largely unexplored area, especially as a route for establishing cytometers as quantitative measurement tools. In this talk, I discuss principles of UQ adapted to cytometry measurements in the context of harmonization of measurements across instruments and other metrological issues.
10:30
20 November 2024
Ballroom B
John Nolan, CEO, Cellarcus Biosciences, Inc., United States of America
Single Vesicle Measurements and the Optimization and Validation of EV Production and Manufacturing
Extracellular vesicles (EVs) are involved in diverse physiological and pathological processes and have attracted attention as potential therapeutics. Sources of potential therapeutic EV preparations include EVs from native or engineered cells in culture or from biofluids, and this source material might be fractionated to enrich EVs relative to other biofluids, and the resulting enriched EVs may be further modified, for example by loading with cargo or surface functionalization with a target molecule. In all cases the functional activities of the resulting EV preparations are presumed to stem from the individual EVs, and their molecular cargo, present with thin the preparation, but direct measurements of these EVs can be challenging. Single EV analysis methods, including single vesicle flow cytometry, can provide measurements of EV concentrations and cargo with the molecular resolution and analytical rigor to support therapeutic EV development and application. In this presentation I’ll review the state of the science of single EV measurement, applications in EV production and manufacturing, and highlight outstanding challenges for the field.
11:00
20 November 2024
Ballroom B
Lynn Pulliam, Professor of Laboratory Medicine and Medicine, University of California-San Francisco, United States of America
Using Extracellular Vesicles to Diagnose and Follow Neurodegenerative Processes
Diagnosing neuro-cognitive disorders has been challenging and doing so without invasive and expensive procedures even more difficult. My lab has been interested in diagnosing chronic cognitive impairment in HIV infection and more recently long COVID using neuronal enriched extracellular vesicles, The goal is to identify liquid biomarkers associated with cognitive impairment and follow these during and after treatment. In addition, studies may identify common and distinct processes in neuro-degeneration that may help in drug interventions.
11:30
20 November 2024
Ballroom B
Terry Morgan, Professor, Oregon Health and Science University (OHSU), United States of America -- Conference Track Co-Chairperson
NanoFACS and Bioassays
12:00
20 November 2024
Ballroom B
Malgorzata Witek, Associate Research Professor, University of Kansas, United States of America
Liquid Biopsy Core (LBC) - Enabling Tools for the Isolation of Liquid Biopsy Markers and Their Molecular Analysis
Liquid biopsies are minimally invasive tests that can be performed frequently, providing “real-time” information on disease status to improve patient outcome. Analyzing different biomarkers present in liquid biopsies, including circulating tumor cells (CTCs), cell-free DNA (cfDNA), and extracellular vesicles (EVs), requires enrichment to select the low abundant disease-associated markers from clinical samples. The LBC provides a diverse range of technologies that are directed at both enriching liquid biopsy markers and their downstream molecular analysis. The LBC uses a combination of microfluidics with full process automation for enriching the full complement of liquid biopsy markers with exquisite analytical figures-of-merit. As examples of utility of LBC technologies, we will present clinical data on identifying the molecular subtypes of breast cancer using EV’s mRNA and monitoring response to therapy in pancreatic cancer via CTCs.
12:30
20 November 2024
Ballroom B
Jimmy Fay, Field Application Scientist, NanoFCM, United States of America
Nano-flow Cytometry for Comprehensive EV Characterization
Nano-flow cytometry has enabled reliable and quantitative measurement of nano-sized objects including extracellular vesicles. In the context of extracellular vesicles, the NanoFCM NanoAnalyzer instrument enables measurement of size, particle count, detection of surface markers, and quantification of cargos in a quick and facile manner. We will describe the principles which enable single particle analysis and several case studies of naturally occurring and engineered EVs. We will also discuss membrane labelling and the detection of internal components relevant to EV research.
13:00
20 November 2024
Exhibit Hall
Networking Buffet Luncheon -- Network with Exhibitors and Colleagues, View Posters
15:00
20 November 2024
Ballroom A
Poster Awards
3 Cash Awards Sponsored by the RSC and Lab-on-a-Chip Journal + Raffle for iPad Drawing