
Extracellular Vesicles (EVs) & Lipid Nanoparticles (LNPs) 2025
Date: Thursday, 15 May 2025 - Friday, 16 May 2025
Location: Miami, Florida
Confirmed Speakers

Andreas Möller, Professor, Director, JC STEM Lab of Personalized Cancer Medicine, Prince of Wales Hospital, Chinese University of Hong Kong

Bowen Li, Assistant Professor, Canada Research Chair in RNA Vaccines and Therapeutics, University of Toronto

Chukwumaobim Nwokwu, Assistant Professor of Biochemistry, Florida Gulf Coast University

Hsueh-Chia Chang, Bayer Professor of Chemical and Biomolecular Engineering, University of Notre Dame

Michael Graner, Professor, Dept of Neurosurgery, University of Colorado Anschutz School of Medicine -- Conference Co-Chairperson

Natalia Tumidajski, Principal Scientist, GMP Site Head, EXOCEL BIO INC.

Owen Fenton, Assistant Professor at the University of North Carolina at Chapel Hill

Susan Belzer, Director of Business Development - US, INOVIQ Limited

Andrew Godwin, Professor and Division Director, Genomic Diagnostics, University of Kansas Medical Center

Brian Feng, CEO, Osem Fluidics

Damien Pearse, Professor, Department of Neurological Surgery; The John M. and Jocelyn H.K. Watkins Distinguished Chair in Cell Therapies, University of Miami Miller School of Medicine

Johnny Zhuang, Business Development & Product Application Scientist, EXODUS BIO

Mousumi Ghosh, Research Associate Professor, The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine

Natasha Sosanya, Research Scientist, US Army Institute of Surgical Research (USAISR)

Shilpa Buch, Professor-Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center

Sven Kreutel, CEO, Particle Metrix, Inc., USA

Banishree Saha, Associate Director, Early Clinical Development, Takeda Pharmaceuticals

Briana Simms, Assistant Professor of Chemistry, University of Cincinnati

Dominique PV de Kleijn, Professor Experimental Vascular Surgery, Professor Netherlands Heart Institute, University Medical Center Utrecht

Kimberly Luke, Vice President, Research and Development, Intuitive Biosciences

Mỹ Mahoney, Professor, Department of Pharmacology, Physiology, and Cancer Biology, Thomas Jefferson University

Noah Malmstadt, Professor, Mork Family Dept. of Chemical Engineering & Materials Science, University of Southern California -- Conference Co-Chairperson

Steve Soper, Foundation Distinguished Professor, Director, Center of BioModular Multi-Scale System for Precision Medicine, The University of Kansas

Varshal Davé, Senior Vice President, Commercial, Exokeryx
SelectBIO Extracellular Vesicles (EVs) and Lipid Nanoparticles (LNPs) 2025 Conference, brings together researchers and industry participants from both academia and companies focusing on studying the biology of EVs and LNPs and focusing on the interface of these fields.
Presentations will explore the latest advances in the EVs field from the study of their biology to their potential as engineered drug delivery vehicles and therarapeutics (targeted delivery of biologics and small molecules).
Additionally, the use of LNPs -- already successfully deployed in the clinic as the Covid-19 vaccine -- as a platform for vaccine and therapy deployment will be the focus of this conference and companies developing LNPs present their research and frame it in the context of clinical utility.
The co-located concurrent track is The Space Summit 2025 and attendees receive Full Access to both tracks running concurrently for maximal scientific exchange and networking.
NCATS has already announced that they will be funding EV research projects in microgravity in low-earth orbit (LEO) and so the fields of EV-research and microgravity research are converging. An excellent opportunity for EV researchers at this conference to engage with LEO experts to discuss and advise on sending research projects into microgravity on-board the International Space Station (ISS) or some of the private space stations coming online soon as a pay-per-use platform for LEO experiments and manufacturing in microgravity environment.
Oral Presentations & Posters
Agenda Topics
You can also present your research in a oral presentation or poster while attending the meeting.
Submit an abstract for consideration now!
Abstract Submission Deadline: December 31, 2024
Exosomes/Extracellular Vesicles (EVs): Biological Investigations of Cargo
EVs as Platform for Drug Delivery: Biologics or Small Molecules
Engineered EVs as a Platform for Therapeutics
Modulation of the Immune Response by EVs
Synthetic LNPs: Characteristics and Properties
LNPs as a Platform for Vaccine and Therapeutic Development
Sponsorship & Exhibition Opportunities
Jeff Fan
Exhibition Manager - SelectBIO
E-mail: Jeff@selectbioconferences.com
3 for 2 Offer on Delegate Registrations
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Gold Sponsors
Exhibitors
Sponsorship and Exhibition Opportunities
If you require any information about exhibiting or sponsoring at one of our events please contact Jeff Fan using the information below:
Jeff Fan, Exhibition Manager
Email: jeff@selectbioconferences.com
Telephone: +1-510-857-4865
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Specialists: SELECTBIO doesn't organise conferences in shipping, accountancy, textiles etc. – just drug discovery and the life sciences. Many of our staff have bioscience qualifications and many years of experience. So, we speak your language and understand your needs.
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Extracellular Vesicles (EVs) & Lipid Nanoparticles (LNPs) 2025 Conference Venue
SelectBIO is delighted to host The Space Summit 2025 and the EV-LNP 2025 Conference at the:
Embassy Suites by Hilton Miami International Airport
3974 NW South River Drive
Miami, FLORIDA 33142
USA
SelectBIO has negotiated discounted hotel rates for stay at this venue at the pricing of US$149 per night (2 Room Suite - 1 King Bed).
The cut-off date for this discounted pricing is April 12, 2025 -- so please make your hotel booking prior to this date to lock-in the discounted pricing.
Check-in 4pm
Check out 11am
Complimentary Miami Airport Shuttle
Complimentary Full Cooked Breakfast
Complimentary Evening Reception with Wine and Beer
Complimentary Wi-Fi
Overnight Self-Parking US$10 per night
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Jeff Fan
Events Manager, SelectBIO
E-mail: Jeff@selectbioconferences.com
SelectBIO has NOT authorized ANY third party company to assist in hotel bookings or reservations for the conference. Please do NOT do business with any third party companies. If in doubt, please contact Jeff Fan immediately to clarify.
Register to this conference and also enjoy the following co-located events at no extra charge.
Training Courses
If you would like to submit a proposal for an oral or poster presentation at this meeting, please fill out the form below required for your submission.
Successful applicants will be provided with all necessary information.
Abstract Content
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Written in English
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Include title, name(s) and affiliation(s) of the authors
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Between 100 - 200 words
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Suitable for direct publication in the proceedings pack and on the website
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Related to the subject of the conference
Agenda Topics
Exosomes/Extracellular Vesicles (EVs): Biological Investigations of Cargo
EVs as Platform for Drug Delivery: Biologics or Small Molecules
Engineered EVs as a Platform for Therapeutics
Modulation of the Immune Response by EVs
Synthetic LNPs: Characteristics and Properties
LNPs as a Platform for Vaccine and Therapeutic Development
Copyrights
The presenting author/person who submitted the abstract assumes full responsibility of the content of the abstract and we assume that all co-authors are aware of this content. Please note that your biography, summary and abstract may be used on this website and conference materials.
Andreas Möller, Professor, Director, JC STEM Lab of Personalized Cancer Medicine, Prince of Wales Hospital, Chinese University of Hong Kong

Andreas Möller Biographical Sketch
Professor Andreas Möller is a trained biochemist and cancer biologist, with >20 years’ experience in cancer research. He is Professor in the Faculty of Medicine at the Chinese University of Hong Kong, supported by the prestigious Global STEM Professor fellowship. In addition, he is the Director of the Jockey Club Laboratory of Precision Cancer Medicine at the Li Ka Shing Institute of Health Sciences. Previously, between 2012 and 2023, he led the Tumour Microenvironment Laboratory at the QIMRB Medical Research Institute, Brisbane, Australia. Professor Möller has a strong background in cancer cell biology, extracellular vesicle (EV) biology, hypoxia research, cancer metastasis and cancer immunology. He is Member of the Board of Directors of the International Society of Extracellular Vesicles since 2022, and since 2024 elected Executive Chair for Meetings and Programs as well as one of the founders and Executive Board Member of the Asia Pacific Societies of Extracellular Vesicles.
Andrew Godwin, Professor and Division Director, Deputy Director, KU Cancer Center, University of Kansas Medical Center

Andrew Godwin Biographical Sketch
Andrew K. Godwin, PhD is the Chancellors Distinguished Chair in Biomedical Sciences endowed Professor and the Director of Molecular Oncology in the Department of Pathology at the University of Kansas Medical Center (KUMC). He is a leader in the field of translational research and precision medicine. A native of Lawrence, Kansas, Dr. Godwin graduated with highest distinction from the University of Kansas (KU) with a bachelor’s degree in Cellular Biology. He obtained his PhD in Molecular Biology from the University of Pennsylvania while carrying out his thesis research at Fox Chase Cancer Center (FCCC) in Philadelphia. While at FCCC he had the pleasure of learning from “giants” in their respective fields of cancer-focused science. He was appointed leader of the FCCC’s Cancer Center Support Grant Ovarian Cancer Program in 2008 and served as co-leader of the Women's Cancer Program from 2009 to 2010. He was the founding director of both the Clinical Molecular Genetics/Pathology Laboratory and the Biosample Repository at FCCC since their inception in 1995 and 1999 respectively, and until leaving FCCC.
Dr. Godwin was recruited to KUMC as the director of Molecular Oncology and as the Associate Director for Translational Research within the KU Cancer Center in October 2010 after 26 productive years at FCCC. His engaged participation in the cancer center led to being appointed the Deputy Director in 2013 and his unwavering efforts helped to gain NCI designation in 2012/2017 and comprehensive designation in 2022. He founded the Clinical Molecular Oncology Laboratory, a CLIA-certified, CAP-accredited molecular diagnostics laboratory in 2012. He was appointed the Division Director for Genomic Diagnostic for the KU Health System in 2020. He also founded the Center for Genetics Services and Health Equity, to address health disparities in medical underserved populations regionally and nationally.
Dr. Godwin holds secondary appointments as a Professor in the Department of Internal Medicine, and the Department of Microbiology, Molecular Genetics, and Immunology. He also leads the ovarian cancer research-working group, is a member of the Investigator Initiated Trial Steering Committee, serves as the founding Scientific Director for the Biomarker Discovery Laboratory, the Director of the KU Cancer Center’s Biospecimen Shared Resource, and the KU Medical Center’s Biospecimen Repository Core Facility. He was named the Vice Chair for the Breast Translational Medicine subcommittee of the Southwest Oncology Group in 2018 and was appointed to the National Cancer Institute’s NCTN Core Correlative Sciences Committee in 2021.
Dr. Godwin heads KU’s institutional efforts in precision medicine. In 2016 he founded the Kansas Institute for Precision Medicine which is currently supported by a Phase 2 Centers of Biomedical Research Excellence (P20 GM130423) grant from the NIGMS. He is also a co-founder of a startup company, Sinochips Diagnostics, a private reference laboratory that provides pharmacogenomic testing to provide a road map for which drugs will be most effective for each individual patient.
He was named a Kansas Bioscience Authority Eminent Scholar in 2010 and the University of Kansas School of Medicine Chancellor’s Distinguished Chair in Biomedical Sciences Endowed Professor in 2012. In 2014, Dr. Godwin received the KUMC School of Medicine’s Achievement Award for the mentoring of post-doctorate students. Of his awards, he is most proud of being acknowledged for his years of mentoring. He has mentored over 150 trainees, including high school students, undergraduates, graduate students, postdoctoral associates/fellows, medical students/fellows, visiting scientists, and junior faculty during his academic career. He was awarded the KU Medical Center’s Faculty Investigator Research Award in 2015, the University of Kansas Cancer Center Director’s William Jewell Team Science Award in 2017, the KUCC Director’s Basic Science Award and the Chancellor’s Club Award for Research in 2018, and the Dolph C. Simons, Sr. Higuchi Award in the Biomedical Sciences in 2020 – the state higher education system’s most prestigious recognition for scholarly excellence. Most recently, Godwin was presented with the 2021 KU School of Medicine Excellences in Mentoring (Faculty) award and the 2021 Cancer Center Director’s Award in Mentoring and was named the 2022 Lead Scholar in Biomedical Sciences by the Ewha Womans University in South Korea. Most recently he presented the 2024 Outstanding Mentorship in Pathology Award by the University School of Medicine and Department of Pathology and Laboratory Medicine in recognition of dedication to the mentorship and education of future pathologists.
Dr. Godwin is internationally recognized for his molecular biology/genetic studies of sarcoma (gastrointestinal stromal tumors and Ewing sarcoma), breast and ovarian cancer, and his efforts to help bridge the gap between basic and clinical science to improve patient care. As a result of his efforts, Dr. Godwin has been continuously since his first faculty appointment at the Fox Chase (in 1993) and has secured extramural funding totaling >$250M. He has published over 550 peer-reviewed manuscripts and scholarly review articles (h index = 143; >95,000 citations) and is the author of 4 patent applications.
Banishree Saha, Associate Director, Early Clinical Development, Takeda Pharmaceuticals

Banishree Saha Biographical Sketch
Dr. Banishree Saha serves as the Associate Director and Analytical Lead for Translational Biomarker Assays at Takeda Pharmaceuticals in Cambridge, Massachusetts. She is instrumental in advancing biomarker assay development and spearheading innovative research in gastrointestinal and liver diseases. Renowned for her expertise in extracellular vesicles, especially exosomes, Dr. Saha has made substantial contributions to understanding the role of microRNA cargo in immune responses during liver injury. Her extensive experience in academia and the pharmaceutical industry allows her to significantly influence the field of liver biology and translational research, devising new therapeutic strategies for liver-related ailments.
Bowen Li, Assistant Professor, Canada Research Chair in RNA Vaccines and Therapeutics, University of Toronto

Bowen Li Biographical Sketch
Dr. Bowen Li is a tenure-track Assistant Professor at the Leslie Dan Faculty of Pharmacy, the Institute of Biomedical Engineering, and the Department of Chemistry at the University of Toronto. He is also an Affiliate Scientist at the Princess Margaret Cancer Centre. Dr. Li holds the Tier 2 Canada Research Chair in RNA Vaccines and Therapeutics and the GSK Chair in Pharmaceutics and Drug Delivery. He earned his Ph.D. in Bioengineering from the University of Washington, Seattle, and completed a postdoctoral fellowship under Profs. Robert Langer and Daniel Anderson at MIT. Dr. Li’s lab employs interdisciplinary strategies, including combinatorial chemistry, high throughput platforms, and AI-driven design of experiments, to develop advanced delivery systems for RNA medicines. Dr. Li has authored over fifty publications in top-tier journals, such as Nature Biotechnology, Nature Materials, Nature Biomedical Engineering, Nature Medicine, PNAS, and Science Advances, and holds eight patents. His research has been recognized with awards including the Moderna Fellowship, AAPS Emerging Investigator Award, CSPS Early Career Award, Marsha Morton Early Career Investigator Award, ACS Rising Star in Biological, Medicinal, and Pharmaceutical Chemistry, Gairdner Early Career Investigator Award, J.P. Bickell Medical Research Award, and Connaught New Researcher Award.
Brian Feng, CEO, Osem Fluidics

Brian Feng Biographical Sketch
Brian Feng is the CEO and co-founder of Osem Fluidics. He received a BS in Biomedical Engineering, MS in Materials Engineering, and PhD in Materials Science from the University of Southern California. His current focus is advancing microfluidic solutions for assembling lipid nanoparticles at high throughput.
Briana L. Simms, Assistant Professor of Chemistry, University of Cincinnati

Briana Simms Biographical Sketch
Dr. Simms, an HBCU alumna, synthetic polymer chemist, and entrepreneur is currently an assistant professor at the University of Cincinnati. Her research focuses on the design and development of functional biomaterials that address public health challenges. Her ultimate goal is to move biomaterials from the benchtop and into the communities that need them most, all while empowering the next generation of STEM scholars.
Chukwumaobim Nwokwu, Assistant Professor of Biochemistry, Florida Gulf Coast University

Chukwumaobim Nwokwu Biographical Sketch
Dr. Chukwumaobim Nwokwu completed his graduate studies in Molecular Science and Nanotechnology. He is a widely published author, with a broad interdisciplinary focus in the fields of applied genomics, cancer therapeutics, nanomedicine, advances in exosome and extracellular RNA (exRNA) technology, biomedical engineering and device design. Dr. Nwokwu is an Assistant Professor of Biochemistry and Head of the Biomimetics Modeling (BM2) Lab at Florida Gulf Coast University.
Damien Pearse, Professor, Department of Neurological Surgery; The John M. and Jocelyn H.K. Watkins Distinguished Chair in Cell Therapies, University of Miami

Damien Pearse Biographical Sketch
Dr. Pearse is a Professor at The Miami Project to Cure Paralysis, Department of Neurological Surgery at the University of Miami Miller School of Medicine. Dr. Pearse is also a Research Health Scientist at the Bruce Carter Miami VA Healthcare System. Dr. Pearse received his undergraduate degree in Biotechnology and doctorate in Neuroscience from Griffith University, Queensland, Australia. Following a postdoctoral fellowship at The University of Miami, Dr. Pearse joined the faculty. Dr. Pearse was an associate in the Christopher and Dana Reeve Foundation Consortium from 2000 to 2006 and in 2005 received the Erica Nader Award for being the Outstanding Investigator in SCI Research from the American Spinal Injury Association. Dr. Pearse is currently The John M. and Jocelyn H.K. Watkins Distinguished Chair in Cell Therapies where his work is focused on the translation of autologous cell therapies for neural repair following spinal cord injury and other neurological disorders.
Dominique PV de Kleijn, Professor Experimental Vascular Surgery, Professor Netherlands Heart Institute, University Medical Center Utrecht

Dominique de Kleijn Biographical Sketch
Prof. Dr. Dominique PV de Kleijn is molecular biologist and chemist and professor of Exp. Vascular Surgery at UMC Utrecht and professor at the Netherlands Heart Institute. From 2012 to 2016, he was Research Professor of Surgery at NUS/NUHS and preclinical director of the Cardiovascular Research Institute (CVRI) Singapore. He was until 2016 professor of Cardiovascular Immunology and co-chair of Experimental Cardiology at UMC Utrecht. Since 1997 he is coordinating cardiovascular research from basic science, animal myocardial infarction and atherosclerotic studies (pig and sheep) towards clinical biobanking studies. His research interests are: The innate immune system in cardiovascular disease and atherosclerosis & Biomarkers predictive for primary & secondary events with a focus on plasma extracellular vesicles. He has more then 275 publications and a H-factor of 78.
Hsueh-Chia Chang, Bayer Professor of Chemical and Biomolecular Engineering, University of Notre Dame

Hsueh-Chia Chang Biographical Sketch
Professor Hsueh-Chia Chang is the Bayer Professor of Chemical and Biomolecular Engineering at the University of Notre Dame. He has been at Notre Dame since 1987 and has served as the department chair and the director of the Center for Microfluidics and Nanofluidics. His research is in the area of micro/nanofluidics and diagnostics, particularly integrated devices for the isolation and characterization of exosomes and other nanocarriers. His research has resulted in 19 patented technologies, eight of them have been licensed by four startups: Cubed Laboratories, AgenDx, ImpeDx and Aopia Biosciences. Products developed from these IPs include CRDTM Botrytis Detection kit by CubedLabs and NanoExTM exosome purification technology by Aopia Bio. Professor Chang served as the Chief Scientific Advisor of FCubed LLC (predecessor of CubedLabs) for 4 years. He co-founded Aopia Bio in 2019 and currently serves as its interim CTO and Board member. Professor Chang has published more than 300 papers and has a Google h-index of 79. He is the co-author of a seminal book in microfluidics: "Electrokinetically Driven Microfluidics and Nanofluidics" by Cambridge University Press in 2009. Professor Chang founded a new journal, Biomicrofluidics of the American Institute of Physics, in 2006 and served for 12 years as its Chief Editor. More than 35 PhD and post-doc students from his laboratory have embarked on academic careers as tenure-track professors in all engineering and science disciplines over 5 continents (15 in the US), including Duke, Imperial, Johns Hopkins etc. They include a chancellor, a Provost, three Department Chairs, four endowed chairs and five NSF Career Awardees. Industrial alumni from his group hold manager/director positions at Merck, Gilead, Alcon, Genentech and other major biotech and world-leading microfluidic companies. Professor Chang is a fellow of APS, NAI and AIMBE. His microfluidics work has been recognized by the Frenkiel Award from APS, the Lifetime Achievement Award from AES and the 1st Source Bank Commercialization Award. He has also won the Notre Dame Provost Research Achievement Award and was recognized at half time during the 2024 game against Stanford.
Johnny Zhuang, Business Development & Product Application Scientist, EXODUS BIO

Johnny Zhuang Biographical Sketch
Johnny Zhuang, a Business Development & Product Application Scientist at EXODUS BIO since March 2023, plays an important role in the development and application of the EXODUS - automatic exosome isolation system. He obtained his Bachelor of Science from Hong Kong Baptist University and his Master of Science in Biotechnology from Georgetown University. Johnny's diverse experience includes significant roles in research and product application at Charles River Laboratories, as well as in research at NOVAVAX INC and Nantomics LLC.
Kimberly Luke, Vice President, Research and Development, Intuitive Biosciences

Kimberly Luke Biographical Sketch
As one of the founding members of Intuitive Biosciences, Dr. Luke has been leading the company’s research efforts in infectious disease diagnostics for over 13 years. She completed her PhD studying influenza virus at the University of Wisconsin-Madison and has worked at small biotech companies and larger pharma companies like Pfizer. Her research has focused on utilizing novel immunoassay platforms for multiplex biomarker detection for bacterial and viral infections in humans and animals, including tuberculosis, HIV, and SARS CoV-2, and she is the primary inventor on several issued and pending worldwide patents. With Intuitive Biosciences, she has been awarded several NIH contracts focused on the development of improved HIV diagnostics. Most recently her work has focused on extracellular vesicle (EV)-based lateral flow immunoassay that overcomes the limitations of current point-of-care HIV testing technologies.
Michael Graner, Professor, Dept of Neurosurgery, University of Colorado Anschutz School of Medicine

Michael Graner Biographical Sketch
Michael Graner received his PhD in Biochemistry from the University of Illinois followed by post-doctoral and research faculty work at the University of Arizona, shifting gears from the Drosophila extracellular matrix to cancer immunotherapy. He then took at faculty position at Duke University’s Tisch Brain Tumor Center, followed by his current position as Professor in Neurosurgery at the University of Colorado Denver (Anschutz Medical Campus). He is also a member of the University of Colorado Cancer Center, the Colorado Clinical and Translational Sciences Institute, the MAVRC Program, and holds a Visiting Professorship Appointment at the Shenzhen Third People’s Hospital (China) and an adjunct faculty appointment at Colorado State University. Graner has a long-standing interest in cell stress responses, which led to cancer vaccine development (including one in clinical trials), which somehow led to the world of extracellular vesicles (EVs). His lab currently concentrates on signaling mechanisms involving EVs, in particular the transfer of stressed phenotypes from stressed tumor cells to unstressed ones via EVs.
Mousumi Ghosh, Research Associate Professor, The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine

Mousumi Ghosh Biographical Sketch
Dr. Ghosh, a Research Associate Professor at The Miami Project to Cure Paralysis in the Department of Neurological Surgery at the University of Miami Miller School of Medicine, is at the forefront of developing innovative therapies for spinal cord injury. Her research focuses on reprogramming microglia and macrophages into neuroprotective and pro-regenerative phenotypes to overcome neurological dysfunction and promote recovery, using clinically relevant rodent models.
Dr. Ghosh earned her Ph.D. in Biochemistry from the University of Calcutta, India, and joined the faculty at the Miami Project after completing her postdoctoral training at the University of Miami. Her work has earned prestigious accolades, including the Sam Schmidt Paralysis Foundation Award and the American Spinal Injury Association’s Spinal Cord Injury Research Award, for groundbreaking contributions to 3D modeling of glial interactions in the injured CNS. Currently, her research delves into modulating microglial exosomal cargo to enhance their anti-inflammatory and pro-regenerative potential, paving the way for novel therapeutic strategies in CNS repair.
Mỹ Mahoney, Professor, Department of Pharmacology, Physiology, and Cancer Biology, Thomas Jefferson University

Mỹ Mahoney Biographical Sketch
Dr. Mahoney is a professor with dual appointments in the Departments of Pharmacology, Physiology, and Cancer Biology and Otolaryngology-Head and Neck Surgery, at Thomas Jefferson University in Philadelphia, PA. She is also a member of the Sidney Kimmel Comprehensive Cancer Center. She received a PhD in Molecular and Cellular Biology from the University of Massachusetts at Amherst and post-doctoral trainings in Cardiovascular Biology at Parke-Davis Pharmaceuticals and in Dermatology at the University of Pennsylvania. Dr. Mahoney is part of the leadership team for the Squamous Cell Carcinoma Tumor Ecology and Microenvironment (STEM) Research Group and is a Co-Director of the Jefferson EV Talks. She is a Board member of the International Society for Extracellular Vesicles and a Co-Director of Meetings for the American Society for Intercellular Communication. The goal of Dr. Mahoney’s laboratory is to understand, at the molecular and cellular level, how normal signaling events during tissue morphogenesis and development are subverted for pathogenic signaling during malignant transformation and disease progression. She focuses on intercellular communication including membrane trafficking, extracellular vesicle biogenesis, and cytokine release.
Natalia Tumidajski, Principal Scientist, GMP Site Head, EXOCEL BIO INC.

Natalia Tumidajski Biographical Sketch
Natalia Tumidajski is a Principal Scientist and GMP Site Head at ExoCel Bio in Doylestown, Pennsylvania with a background in regenerative medicine, stem cell research, and exosome technology, Her work focuses on developing innovative solutions for skin health, anti-aging, hair rejuvenation, and wound healing. In her current role, Natalia leads the development and management of a state-of-the-art facility specializing in regenerative medicine. She oversees GMP and R&D laboratories, ensuring compliance with regulatory standards and driving innovative research initiatives. Her leadership extends to strategic site management, experimental design, and the development of novel products. Natalia is also an active presenter at national conferences, sharing her findings and contributing to the scientific community.
Natalia’s lab is dedicated to optimizing GMP manufacturing of exosomes, conducting in-depth characterization studies—including mRNA and growth factor expression profiling—and assessing the effects of lyophilization on extracellular vesicle stability. The team is also evaluating advanced 3D cell culture systems and exploring the effects of microgravity and hypoxia on exosome yield and potency, aiming to enhance their therapeutic potential for regenerative medicine.
Natasha Sosanya, Research Scientist, US Army Institute of Surgical Research (USAISR)

Natasha Sosanya Biographical Sketch
Currently, I am a Principal Investigator at the US Army Institute of Surgical Research (USAISR) where I utilize several animal models of injury to (1) study the relationship between stress and pain, (2) elucidate potential pain biomarkers, (3) explore potential drug targets that function to reduce injury-induced pain and (4) test the efficacy and safety of potential pain therapeutics. Previously, I obtained my PhD in Molecular Neuroscience at the University of Texas at Austin where I determined how specific RNA Binding Factors (such as microRNAs) regulate ion channel expression in normal and disease states. I am currently applying my eleven years of graduate and post-doctoral training in neuroscience, pain, and molecular techniques to elucidate and study the underlying causes of pain and the best potential treatment options.
Noah Malmstadt, Professor, Mork Family Dept. of Chemical Engineering & Materials Science, University of Southern California

Noah Malmstadt Biographical Sketch
Noah Malmstadt is Professor at the University of Southern California. He received a BS in Chemical Engineering from Caltech and a PhD in Bioengineering from the University of Washington. Following postdoctoral work at UCLA, he joined the Mork Family Department of Chemical Engineering and Materials Science at USC in 2007. Malmstadt is the recipient of a 2012 Office of Naval Research Young Investigator award. His research focuses on microfluidic strategies to facilitate material fabrication and biophysical analysis. He has pioneered the integration of ionic liquids as solvents in droplet microreactors and the application of microfluidic systems to synthesizing biomimetic cell membranes. Microfluidic analytical techniques he has developed include methods for measuring the permeability of cell membranes to druglike molecules and techniques for measuring ionic currents through membrane proteins.
Owen Fenton, Assistant Professor at the University of North Carolina at Chapel Hill

Owen Fenton Biographical Sketch
Owen S. Fenton, Ph.D. is an Assistant Professor at the University of North Carolina at Chapel Hill. The Fenton laboratory is located in the Division of Pharmacoengineering and Molecular Pharmaceutics within the University of North Carolina at Chapel Hill Eshelman School of Pharmacy. Research in the Fenton laboratory focuses on the development of mRNA-based nanomedicines for the study, prevention, and treatment of disease.
Shilpa Buch, Professor-Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center

Shilpa Buch Biographical Sketch
I am currently a Professor & Executive Vice Chair for Research and the Director of the Nebraska Center for Substance Abuse research at the University of Nebraska. I received my PhD in 1982 in Microbiology from Maharaja Sayajirao Univ in Baroda, India and moved to Canada for postdoctoral training. I began my independent research career as an Assistant Professor at the Hospital for Sick Kids, Toronto, following which, I moved to Kansas University and embarked on a research area focused on understanding how addictive drugs co-operate with HIV-1 to exacerbate neurological complications. I rose through the ranks at Kansas and in 2007, made a move as a full Professor to University of Nebraska in Omaha. Research approaches used in my lab involve a multi-pronged approach comprising of a variety of complementary model systems ranging from cell cultures to rodent models to the higher more relevant macaque model of SIV pathogenesis. More recently, my research interest is centered on exploring how exosomes act as conduits to transport key signaling mediators (small noncoding RNAs/microRNAs) to distant recipient cells as a means to regulate gene expression and cellular cross talk. I lead an active research program involving collaborations both nationally and internationally, with over 225 peer-reviewed publications. I have consistently held NIH funding throughout my career and continue to serve on NIH study sections.
During my career, I have had the good fortune of being recognized by various national and International societies with the Wybran (2012) and the Distinguished service (2023; 2013) Awards, both of which hold a special meaning for me. I have also been awarded the UNMC Scientist laureate award (2016) in addition to the Kansas City scientist award. Aligning closely with my passion for mentoring, has enabled me to take an active leading role in the Women’s Mentoring Program at UNMC (2015-2017). I have also received the Women in Neuroscience award at the International Society of Neurovirology in 2016. I have played an active role as a Secretary of the Society on Neuroimmune Pharmacology and have been invited as a speaker & as a Chair at various meetings and have also organized several symposia. To further hone my leadership skills, I graduated from the Executive Leadership for Academic Medicine Program that fosters the growth and career trajectories of women leaders nationally.
Steve Soper, Foundation Distinguished Professor, Director, Center of BioModular Multi-scale System for Precision Medicine, The University of Kansas

Steve Soper Biographical Sketch
Professor Soper (since 2016) is a Foundation Distinguished Professor in Chemistry and Mechanical Engineering at the University of Kansas. At KUMC, Prof. Soper holds an adjunct appointment in the Cancer Biology Department and is a member of the KU Cancer Center. He also holds an appointment at Ulsan National Institute of Science and Technology in Ulsan, South Korea, where he is a World Class University Professor.
As a result of his efforts, Prof. Soper has secured extramural funding totaling >$125M, has published over 245 peer-reviewed manuscripts (h index = 70; >17,000 citations); 31 book chapters and 71 peer-reviewed conference proceeding papers, and is the author of 12 patents. He is also the founder of a startup company, BioFluidica, which is marketing devices for the isolation and enumeration of liquid biopsy markers. Soper recently founded a second company, Sunflower Genomics, which is seeking to market a new DNA/RNA single-molecule sequencing platform. His list of awards includes Ralph Adams Award in Bioanalytical Chemistry, Chemical Instrumentation by the American Chemical Society, the Benedetti-Pichler Award for Microchemistry, Fellow of the AAAS, Fellow of Applied Spectroscopy, Fellow of the Royal Society of Chemistry, R&D 100 Award, Distinguished Masters Award at LSU and Outstanding Scientist/Engineer in the state of Louisiana in 2001. Finally, Prof. Soper has granted 50 PhDs and 7 MS degrees to students under his mentorship. He currently heads a group of 15 researchers.
His major discoveries include: (1) Technology for the detection of liquid biopsy markers that can manage a variety of diseases using a simple blood test (test has been demonstrated in multiple myeloma, pediatric acute lymphoblastic leukemia, acute myeloid leukemia, pancreatic, breast, colorectal, prostate, and ovarian cancers); (2) new hardware and assay for the point-of-care diagnosis of acute ischemic stroke; (3) single-molecule DNA and RNA sequencing nanotechnology; and (4) currently working on a home-test for COVID-19 infections (handheld instrument and the associated assay.
Susan Belzer, Director of Business Development - US, INOVIQ Limited

Susan Belzer Biographical Sketch
Susan Belzer is an accomplished biotechnology and clinical diagnostics executive with extensive experience in research, product development, and business strategy. She has held leadership roles in companies such as INOVIQ, MD Biosciences, and Exosome Diagnostics. Throughout her career, she has successfully led cross-functional teams, managed regulatory compliance, and developed innovative diagnostic solutions. Susan is a recognized expert in molecular diagnostics, clinical trial strategies, and laboratory management, with multiple publications and industry awards highlighting her contributions to the field.
Sven Kreutel, CEO, Particle Metrix, Inc.

Sven Kreutel Biographical Sketch
Sven Kreutel studied biology at the University of Hohenheim in Stuttgart, Germany. After his graduation in biology, he received his PhD in microbiology and protein biochemistry at the University of Hohenheim under Prof. Dr. Andreas Kuhn and Dr. Dorothee Kiefer working on the light sensing and signal transduction in photosynthetic bacteria. Since then he worked in different sales and sales manager positions within the life science industry from clinical research over biology to nanoparticle sciences. In 2021 he was appointed as CEO of the American subsidiary of Particle Metrix.
Varshal Davé, Senior Vice President, Commercial, Exokeryx

Varshal Davé Biographical Sketch
Varshal thrives in the commercialization of novel technologies, and strongly believes that innovation accelerates discovery. Prior to joining Exokeryx as SVP Commercial Operations, he most recently served as CEO of ORFLO Technologies, and has held senior commercial roles in several companies, ranging from startups to large conglomerates. He trained under Harold “Skip” Garner at the University of Texas Southwestern Medical Center, and holds a M.S. in Biology from the University of Texas at Arlington and an A.B. in Chemistry from Duke University.
08:00
15 May 2025
Grand Ballroom Foyer
Conference Registration, Materials Pick-Up, Coffee and Networking
09:00
15 May 2025
Havana

Michael Graner, Professor, Dept of Neurosurgery, University of Colorado Anschutz School of Medicine -- Conference Co-Chairperson
Introduction to the EV, LNP 2025 Conference -- Topics Covered and Synergies Across Disciplines
09:05
15 May 2025
Havana

Noah Malmstadt, Professor, Mork Family Dept. of Chemical Engineering & Materials Science, University of Southern California -- Conference Co-Chairperson
Introduction to LNPs -- Why are LNPs Gaining Attention and a Survey of Applications
09:10
15 May 2025
Havana
Session Sub-Title: Extracellular Vesicles 2025 -- Progress in Research, Diagnostics & Therapeutics Development
Chairperson: Professor Michael Graner, University of Colorado - Anschutz Medical Center
09:15
15 May 2025
Havana
Keynote Presentation

Steve Soper, Foundation Distinguished Professor, Director, Center of BioModular Multi-Scale System for Precision Medicine, The University of Kansas, USA
Label-Free Detection and Identification of Single Protein Molecules Harvested from Extracellular Vesicles
Extracellular vesicles contain a wide range of different types of molecular cargo, such as lipids, carbohydrates, nucleic acids, and proteins. However, due to their small size (~150 nm for exosomes), the number of molecules is limited. For example, there can be ~20,000 protein molecules per EV vesicle. Because the proteome is estimated to consist of >100,000 different proteins, there are less than a single copy of a particular protein per EV particle. As such, specialized techniques have been developed to search for these rare proteins, such as OLink, which use the proximity ligation assay and quantitative PCR to detect these rare events. Challenges include the complicated and long workflow and the use of expensive antibodies. To address this issue, we are developing a nanotechnology to quantify and identify single protein molecules based on peptide fingerprinting.
Resistive Pulse Sensing (RPS) is a label-free and single-molecule detection approach that requires simple instrumentation to implement and as such, can be mobilized to be integrated into in vitro diagnostic assays for not only detecting but identifying key disease-associated biomarkers with high analytical sensitivity, such as proteins. Thus, RPS is a logical choice for coupling with liquid biopsy markers for the precision management of a variety of diseases due to the significant mass limits imposed on any assay in which a liquid biopsy marker is used. We have developed a unique measurement modality and sensor technology (dual in-plane nanopore sensor) that couples RPS to nanoscale electrophoresis. Not only does the sensor generate the typical RPS measurement parameters, but also the molecular-dependent electrophoretic mobility, which we call the time-of-flight (ToF). The RPS parameters coupled with the ToF and machine learning lead to high detection efficiency and classification accuracy of single molecules harvested from liquid biopsy markers, including proteins. Our devices, which are made from plastics via high-scale production modalities (injection molding), consist of channels with dimensions ranging from 1 to 100 nm (effective diameter) that are 10’s of microns in length.
In this talk, I will discuss the operational parameters and unique applications of our dual in-plane nanopore sensor for peptide fingerprinting of single protein molecules. The technology I will discuss involves the use of an immobilized nanoscale enzymatic reactor (INER) containing a proteolytic enzyme to digest the input protein molecule into peptides that generates a fingerprint of the protein by the nanoscale electropherogram pattern produced. Operational characteristics of this sensor for peptide fingerprinting will be shared.
09:45
15 May 2025
Havana
Big-Pharma Presentation

Banishree Saha, Associate Director, Early Clinical Development, Takeda Pharmaceuticals, United States of America
Exosomes as Biomarkers: A New Frontier in Medical Diagnostics
Exosomes represent a promising frontier in medical diagnostics, offering unique capabilities as biomarkers for various diseases, including liver disease. These nano-sized vesicles, secreted by cells, carry proteins, lipids, and nucleic acids that reflect the physiological status of their parent cells. Emerging studies underscore their role in intercellular communication and their potential in disease detection and monitoring. This talk will explore recent advancements in exosome isolation and characterization techniques and their application in diagnosing cancer, neurological disorders, infectious diseases, and liver disease. We will discuss the challenges and future directions for integrating exosome-based diagnostics into clinical practice, aiming to enhance precision medicine through non-invasive and early detection methods.
10:15
15 May 2025
Coconut Grove
Mid-Morning Coffee Break and Networking with Exhibitors
10:45
15 May 2025
Havana

Sven Kreutel, CEO, Particle Metrix, Inc., USA and Germany
Characterization of Extracellular Vesicles and Other Biological Nanoparticles Using Nanoparticle Tracking Analysis (NTA)
Nanoparticle Tracking Analysis (NTA) has emerged as a fast and vital characterization technology for Extracellular Vesicles (EVs), Exosomes and other biological material in the size range from 30 nm to 1 µm. While classic NTA scatter operation feeds back the size and total particle concentration, the user typically cannot discriminate whether the particle is a vesicle, protein aggregate, cellular trash or an inorganic precipitate. The fluorescence detection capabilities of f-NTA however enables the user to gain specific biochemical information for phenotyping of all kinds of vesicles and viruses. Alignment-free switching between excitation wavelengths and measurement modes (scatter and fluorescence) allow quantification of biomarker ratios such as the tetraspanins (CD63, CD81 and CD9) within minutes. Furthermore, specific colocalization studies using c-NTA gives a deeper understanding of the composition of biomarker on single particle.
11:15
15 May 2025
Havana

Kimberly Luke, Vice President, Research and Development, Intuitive Biosciences, USA
EV or Virus? Using Common Features of Viruses and EVs for a Novel HIV At-Home Lateral Flow Test
The similarities between extracellular vesicles (EVs) and viruses make them challenging to distinguish and separate into unique populations. A novel lateral flow test has been developed exploiting these shared properties to improve at-home testing for HIV. By targeting EVs released from HIV infected cells (HIV-EVs) and HIV virus particles for immunocapture, which share common transmembrane proteins like tetraspanins, HIV proteins can be concentrated from blood samples in a lateral flow device. Others have described immunocapture of EVs by lateral flow; here we describe capture and lysis for downstream detection of specific HIV cargo for a sensitive antigen-only HIV test. Investigation of EV cargo from HIV+ cell lines and HIV+ volunteers demonstrated that HIV antigens p24 and Nef are detected as EV cargo early in infection making them ideal biomarkers. Development of new antibodies against HIV Nef, characterization of HIV-EV cargo following the MISEV 2023 standards, and detection of these antigens using multiple immunoassays confirms their utility for rapid diagnostics. This work describes the prototype development and testing that may significantly improve the sensitivity of an at-home test format by utilizing EVs as a novel reservoir of HIV antigens.
11:45
15 May 2025
Havana

Johnny Zhuang, Business Development & Product Application Scientist, EXODUS BIO, United States of America
High-Efficiency Isolation of Exosomes and Its Application in Diagnosis and Treatment
Introducing EXODUS, our innovative automatic exosome isolation system, which employs advanced techniques such as periodic negative pressure oscillation and double-coupled harmonic oscillation. This unique integration ensures the high yield and purity of label-free exosomes. Widely utilized in research for disease biomarker discovery, EXODUS also offers a large-scale model, the EXODUS-T, specifically designed to cater to industrial production demands.
12:15
15 May 2025
Havana
Keynote Presentation

Andrew Godwin, Professor and Division Director, Genomic Diagnostics, University of Kansas Medical Center, United States of America
Innovations to Detect Ovarian Cancer at its Origin: Combining Cancer Biology and Extracellular Vesicle-based Diagnostic Tools
“Ovarian cancer” is a double misnomer, as it is neither a single “cancer,” nor, strictly speaking, always “ovarian” in origin. Histological subtyping reveals a heterogeneous group of neoplasms (low- and high-grade serous, endometrioid, clear cell, and mucinous), each with distinct routes of pathogenesis and aggressiveness. Although these cancers share an anatomical site at presentation, most originate in tissues other than the ovary. High grade serous ovarian cancer (HGSOC) is the most clinically significant subtype, comprising ~70% of cases and 90% of deaths. When localized to the ovaries and fallopian tube (FT), 90% of HGSOC cases can be cured, but only 22% of cases present at this early stage, contributing to its high mortality. HGSOC is thought to originate from the FT, specifically the secretory epithelial cells, and progresses from p53 signatures to a precursor lesion called serous tubal intraepithelial carcinoma (STIC). Evolutionary analyses suggest a window of 6 to 7 years between development of a STIC and initiation of cancer, followed by rapid metastasis. Thus, there is a significant time for screening, especially in high-risk populations (e.g., BRCA1/2 mutation carrier). This new paradigm emphasizes the need to identify biomarkers capable of distinguishing benign or dormant precursor lesions from those that may progress and apply these markers to sensitive liquid-based biopsy approaches. Discussed are biological approaches to characterize small extracellular vesicles (sEVs) associated with malignant transformation of fallopian tube epithelium cells to the discovery of exo-biomarkers for early detection; potentially while still confined to the FT.
12:45
15 May 2025
Atrium
Networking Lunch in the Atrium
13:59
15 May 2025
Havana
Session Sub-Title: EV-based Therapeutics -- Status Update, ca. 2025
14:00
15 May 2025
Havana
Keynote Presentation

Damien Pearse, Professor, Department of Neurological Surgery; The John M. and Jocelyn H.K. Watkins Distinguished Chair in Cell Therapies, University of Miami Miller School of Medicine, USA
The Protective and Regenerative Effects of Schwann Cell EVs
Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication, influencing the behavior of target cells both locally and remotely by delivering RNA, lipid and protein cargos. EVs can be collected in large quantities from cultured human and rodent Schwann cells (SCEVs), offering potential therapeutic benefits for neurological disorders and neurotrauma wherein the transplantation of the parent cell has shown efficacy. SCEVs can promote immunomodulation, reduce inflammation, prevent cell death, alter cell differentiation, and enhance axon growth and neuroplasticity. We have been interested in the development and use of SCEVs as a potential therapeutic for providing cytoprotection and stimulating regeneration, particularly in the context of improving outcomes in models of CNS injury and disease. Here we will present our latest work with SCEVs in neural cell-based assay systems and in in vivo paradigms of neurological injury and disease.
14:30
15 May 2025
Havana

Mousumi Ghosh, Research Associate Professor, The Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami Miller School of Medicine, United States of America
Investigating the Therapeutic Potential of Modified Microglial Exosomes in Spinal Cord Injury Repair
Traumatic spinal cord injury (SCI) results in severe, long-term disability and high mortality, with limited effective treatments to mitigate secondary tissue damage or restore function. Although cell transplantation shows promise in experimental SCI, numerous challenges have restricted its clinical application. Recently, exosomes—nanoscale vesicles secreted by all cell types—have gained attention as a potential therapeutic approach for CNS injuries and diseases due to their exceptional bioavailability, safety, and stability. Exosomes carry bioactive molecules, including miRNAs and proteins, that promote cell survival, axon regeneration, and anti-inflammatory effects.
Microglia, the CNS's innate immune cells, play a crucial role in SCI repair, but their exosomal contributions remain underexplored. In this study, we evaluated the neuroprotective and pro-regenerative effects of phenotypically modulated microglia-derived exosomal vesicles (pmMGEVs), preconditioned using a combination of anti-inflammatory cytokines and small molecule modulators. In vitro, pmMGEVs were readily taken up by neurons, significantly enhancing neuronal survival and growth on inhibitory substrates such as chondroitin sulfate proteoglycan. Systemic administration of pmMGEVs in clinically relevant murine models of moderate SCI improved locomotor function compared to naïve MGEVs.
Multi-omics analysis of pmMGEV cargo, compared to naïve MGEVs, identified specific miRNAs and transcription factors likely driving these therapeutic effects. These findings suggest that pmMGEVs represent a promising, cell-free therapeutic approach for enhancing SCI repair and functional recovery.
15:00
15 May 2025
Havana

Natasha Sosanya, Research Scientist, US Army Institute of Surgical Research (USAISR)
Identifying Stress-Exacerbated Thermal-Injury Induced Pain Biomarkers
Using a model of Combat and Operational Stress Reaction (COSR), our lab recently showed that exposure to an unpredictable combat stress (UPCS) procedure prior to a thermal injury (TI) increases pain sensitivity in male rats. Additionally, our lab has recently shown that circulating extracellular vesicle-microRNAs (EV-miRNAs), which normally function to suppress inflammation, were down-regulated in a male rat model of neuropathic pain. In this current study, female and male rats were exposed to UPCS followed by TI and then evaluated for changes in circulating EV-miRNAs.
Adult female and male Sprague Dawley rats were exposed to the UPCS procedure for either 2 or 4 weeks. Groups consisted of the following: non-stress (NS), stress (S), NS+TI, and S+TI. Mechanical sensitivity was measured, and plasma was collected at relevant timepoints to include baseline, during UPCS exposure, and after TI. EV-miRNA isolation was then performed, followed by small RNA sequencing and subsequent data analysis.
UPCS exposure alone resulted in mechanical allodynia in both males and female rats at specific time points. Thermal-injury induction occurring at peak UPCS resulted in increased mechanical allodynia in the injured hind paw compared to thermal-injury alone. Differential expression of the EV-miRNAs was observed between the NS and S groups as well as between NS+TI and S+TI groups. Consistent differences in EV-miRNAs are detectable in both COSR as well as during the development of mechanical sensitivity. Therefore, EV-miRNAs may potentially serve as key regulators, biomarkers, and targets in the treatment of COSR and thermal-injury induced mechanical sensitivity.
15:30
15 May 2025
Havana

Susan Belzer, Director of Business Development - US, INOVIQ Limited, USA
Developing Diagnostic Assays Using Biomarkers in EVs
Exosomes, nanoscale extracellular vesicles, offer significant potential for early disease diagnosis and monitoring therapeutic responses. However, integrating exosome-based diagnostics into clinical laboratories presents several challenges. A major hurdle is bridging the gap between academic research, test development, and the practical demands of clinical laboratory operations. Drawing from my experience in translating research into clinical diagnostics, this presentation aims to help others navigate this process.
16:00
15 May 2025
Coconut Grove
Mid-Afternoon Coffee Break and Networking in the Exhibit Hall
17:00
15 May 2025
Havana
Keynote Presentation

Dominique PV de Kleijn, Professor Experimental Vascular Surgery, Professor Netherlands Heart Institute, University Medical Center Utrecht, The Netherlands
Plasma Extracellular Vesicles in Reducing Hospital Referrals for Chest Pain Patients
Chest pain induced by exercise or stress is very common in USA and EU with approximately 500,000 GP visits/year in the Netherlands. Based on a better safe than sorry strategy, the GP refers about half of these patients suspected for obstructive coronary artery disease for a coronary CT scan to the hospital. After the scan, only 20% of male and 10% of female patients have indeed obstructive coronary artery disease. To increase the efficiency and reduce costs and time to scan, we developed a blood test based on plasma extracellular vesicle (EV) proteins that can, with an AUC of 0.8, determine which chest pain patients have obstructive coronary disease or not. To withhold chest pain patients from referral for a CT scan, however, an AUC 0.8 is not sufficient. For this, we now investigate the combination of microfluidic EV isolation with existing proteomics technologies of OLINK and EvoSep One LCMSMS and clinical parameters to safely exclude chest pain patients for referral for CT-scan.
17:30
15 May 2025
Havana
Keynote Presentation

Hsueh-Chia Chang, Bayer Professor of Chemical and Biomolecular Engineering, University of Notre Dame, United States of America
Large-Scale Isolation and Detection of Nanocarriers Biomarkers with Nanotechnologies: Parsing Heterogeneity
Intense research in the last 2 decades have shown that nucleic acid and protein biomarkers are secreted from parent cells as cargoes of nanocarriers like lipoproteins, exosomes (or small extracellular vesicles sEVs) and ribonucleoproteins. These nanocarriers range from 10 nm to 1 micron in size and are extremely heterogeneous. It is now clear that the heterogeneity is due to the different biogenesis pathways of the nanocarriers and hence each nanocarrier variant contains cell-specific and disease-specific biomarkers. I will summarize the results from my lab on the fractionation of these nanocarriers and on profiling of their colocalized protein and miRNA cargoes. Due to the nanodimension of the carriers, we utilize low-cost nanoporous membrane technologies with nanopores of comparable size. The goal is to develop a pan-disease screening platform for the annual blood test, after all the biomarkers are discovered. I will report preliminary data with clinical samples from 100-patient cohorts. These technologies were also used for nanocarrier biomarker discovery, in conjunction with NanoString, NGS and proteomic/lipidomic mass spectrometry. The large-volume data offered by massively parallelized versions of our technologies are ideal for regression studies with Machine Learning. Some of these technologies are being commercialized by Aopia Biosciences, a company I co-founded.
18:00
15 May 2025
Atrium
Networking Reception with Beer & Wine
19:00
15 May 2025
Atrium
Close of Day 1 of the Conference
08:00
16 May 2025
Coconut Grove
Morning Coffee and Networking in the Exhibit Hall
08:30
16 May 2025
Havana

Owen Fenton, Assistant Professor at the University of North Carolina at Chapel Hill, United States of America
Investigating the Role of Hypoxia on Lipid Nanoparticle Mediated mRNA Delivery
Hypoxia is a common hallmark of human disease that is characterized by abnormally low oxygen levels in the body. While the effects of hypoxia on many small molecule-based drugs are known, its effects on several classes of next-generation medications including messenger RNA therapies warrant further study. Here, we provide an efficacy and mechanism driven study that details how hypoxia impacts the cellular response to messenger RNA Lipid Nanoparticle therapies. In brief, our work provides a comparative analysis as to how various states of oxygenation impact lipid nanoparticle-delivered mRNA expression, cellular association, endosomal escape, and intracellular ATP concentrations following treatment with 4 different lipid nanoparticle chemistries, 3 different cell lines, 4 different oxygenation ratios, and 6 different reoxygenation conditions. In brief, our results demonstrate that hypoxia decreases the efficacy of mRNA Lipid Nanoparticles by upwards of 80%. Further, our results also suggest that these efficacy decreases correlate with decreased intracellular ATP levels in hypoxic cells. Taken collectively, our results suggest that cellular and tissue oxygen levels may be an important factor to consider when developing messenger RNA Lipid Nanoparticle based therapies.
09:00
16 May 2025
Havana
Keynote Presentation

Noah Malmstadt, Professor, Mork Family Dept. of Chemical Engineering & Materials Science, University of Southern California, USA -- Conference Co-Chairperson
LNPs -- A Deep Dive
09:30
16 May 2025
Havana

Briana L. Simms, Assistant Professor of Chemistry, University of Cincinnati, United States of America
Design, Synthesis, and Characterization of Structurally Tunable Lipid Nanoparticles (LNPs) for Transdermal Delivery
Lipid nanoparticles (LNPs) have emerged in the literature as a viable vehicle for therapeutic delivery, gaining significant popularity over the last 30 years. LNPs are composed of a mixture of biocompatible lipids, which results in variance in nanoparticle properties such as size, surface charge density, or rigidity. This heterogeneity within the formulation can decrease targeting efficiency in drug delivery applications. To address these challenges, we have developed a library of structurally tunable lipid nanoparticles (stLNPs) formed by the self-assembly of a single synthetic lipid with modifiable features. This results in uniform physical (i.e. size and morphology) and mechanical (i.e. rigidity, flexibility, and bilayer morphology) properties across the nanoparticle formulation and allows for the specific selection of LNP properties ideal for the application. With a long-term goal of advancing these materials towards clinical application for targeted transdermal delivery, we have designed and characterized synthetic lipids comprised of a poly amidoamine (PAMAM) dendron chemically linked to an acyl tail group. We have incorporated tunable features that afford nanoparticles with a range of properties. To assess the feasibility of these materials in transdermal delivery, we have treated human skin with the stLNPs and evaluated their impact on skin physiology and penetration depth. The work described herein will provide a framework for the strategic design and synthesis of LNPs for targeted drug delivery. This work will also allow us to better understand how small structural changes to materials can influence the final fate of LNPs in vivo.
10:00
16 May 2025
Coconut Grove
Mid-Morning Coffee Break and Networking in the Exhibit Hall
10:30
16 May 2025
Havana

Varshal Davé, Senior Vice President, Commercial, Exokeryx, United States of America
Demeter EVPrep: Where Microchips Meet Biotech to Deliver Automated, Reproducible EV Isolation
Standard EV isolation methods use a single physical property to isolate EVs, forcing researchers to choose between recovery and purity. Demeter EVPrep is different. Demeter EVPrep uses dielectrophoresis, ingenuity we’ve adapted from the semiconductor industry. This chip-based technique isolates EVs by exploiting their unique dipole moments, which are defined by both their size and their composition. Because Demeter EVPrep leverages these two physical properties simultaneously, it selectively isolates EVs to deliver greater recovery and purity than other techniques. And Demeter EVPrep is reproducible by design. Because it is fully automated with no hands-on steps, you get the same output every single time. Learn how Demeter EVPrep's superior performance and reproducibility can accelerate your discovery, saving you time and money.
11:00
16 May 2025
Havana

Brian Feng, CEO, Osem Fluidics, United States of America
Advancement of Mixing Methods for Assembly of Nucleic Acid-Lipid Nanoparticles
The mixing of nucleic acid and lipid nanoparticles has evolved from simple manual pipette mixing to microfluidics, offering increased control over mixing rate, time, and flow conditions for the assembly of lipid nanoparticles. Although microfluidics is the gold standard for assembling LNPs, there are improvements to current methods that can increase the adoption of microfluidics. In this talk, we will introduce a novel microfluidics format for producing desirable lipid nanoparticles that makes it accessible to all researchers worldwide.
11:30
16 May 2025
Havana

Bowen Li, Assistant Professor, Canada Research Chair in RNA Vaccines and Therapeutics, University of Toronto, Canada
AI-Guided Development of Lipid Nanoparticles for mRNA Delivery
The ability to transfect selective cell types within the targeted tissue in vivo is critical for potential therapeutic applications of mRNA. Although great advances have been made in mRNA vaccines, the ideal chemical and formulation composition of lipid nanoparticles (LNPs) for extra-hepatic delivery of nucleic acids are largely unknown. The traditional development of new lipids and formulations has been challenging, given the complexity of biological systems. In this talk, I will introduce an AI-guided high-throughput platform where thousands of chemically diverse libraries of lipid-like materials can be rapidly synthesized using multicomponent reactions and formulated into LNPs, which can then be screened for tissue- or cell-specific gene delivery. This platform technology increases the diversity of synthetic material structures and facilitates the identification of structure-function relationships.
12:00
16 May 2025
Havana
Keynote Presentation

Andreas Möller, Professor, Director, JC STEM Lab of Personalized Cancer Medicine, Prince of Wales Hospital, Chinese University of Hong Kong
Translation of Extracellular Vesicles from Bench to Bedside
Extracellular vesicles (EVs) present a huge, yet largely untapped resource for disease diagnostics and therapeutics. While significant advancements have been made in bench-based investigations, these have largely not yet been successfully translated to clinical or commercial applications. In the context of cancer, the evaluation of EV cargo composition changes allowed us to develop highly sensitive, liquid biopsy diagnostic and prognostic biomarkers. In this presentation, the translational work, including the validation in retrospective patient cohorts and prospective trials will be discussed. Some of the technical and logistic challenges faced when transitioning bench methods to bedside applications, and their implementation into a hospital setting, will be highlighted.
12:30
16 May 2025
Atrium
Networking Lunch
13:30
16 May 2025
Havana

Natalia Tumidajski, Principal Scientist & GMP Site Head, ExoCel Bio, Pennsylvania Biotechnology Center, United States of America
Space Microgravity and Hypoxia Conditions as a Novel Approach to Enhance Regenerative Potency of MSC-derived Exosomes in Specialized 3D Cell Culture System
Research in microgravity is emerging as a new approach in studying biological systems and designing better treatment options. Recent studies in space have shown that microgravity transforms the behavior of stem cells and has unparallel effects on their growth, proliferation, and secretion of bioactive molecules such as extracellular vesicles. Regardless of continuous advancements in stem cell-based therapies, there are still significant challenges to ensure reproducibility, stability, and maximized potency for biomedical applications. Universal 2D cell culture system fails to mimic a natural environment for stem cells limiting their full capabilities including reduced stemness and deficient cell to cell interactions. Our approach was to create a natural niche for mesenchymal stem cells where they can cultivate their full biological potential and release the most potent exosomes- significant meditators in cell-to-cell communication via enriched cargo of proteins, lipids, and mRNAs. In this study, using placental MSCs we generated 3D cell models- spheroids with 5th dimensional technology and hydrogel-based organoids. They were then cultured in bioreactors in specialized 3D systems using microgravity, hypoxia, and rotational speed as the main conditions to mimic unique space environment. Upon isolation of exosomes from conditioned media the comprehensive characterization was conducted to validate their enhanced biological activity and regenerative potential. This completely novel and innovative study could ignite a cutting-edge revolution for regenerative applications such as wound healing, tissue engineering, and organ regeneration.
14:00
16 May 2025
Havana

Shilpa Buch, Professor-Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, USA
HIV Tat-Stimulated Microglial Extracellular Vesicles Shuttle Ferroptosis Mediators and Induce Neuronal Injury: Role of Dysregulated Autophagy
Objective: This study investigated how dysregulated autophagy contributes to HIV Tat-induced ferroptosis in microglia, the subsequent release of ferroptotic cargo via microglia-derived extracellular vesicles (MEVs), and their impact on neuronal injury.
Methods: Microglial cells were exposed to HIV Tat, followed by assessments of ferroptosis markers, autophagy-related proteins, and MEV cargo composition using Western blot, qPCR, and immunofluorescence. Pharmacological modulation of ferroptosis (ferrostatin-1, deferoxamine) and autophagy (bafilomycin A1, rapamycin) was employed to delineate mechanistic pathways. MEVs were isolated and characterized from microglial culture supernatants and HIV transgenic rat brain lysates. Primary cortical and hippocampal neurons were treated with Tat-MEVs, and ferroptosis mediators, iron content, mitochondrial dysfunction, synaptic markers, and dendritic spine density were assessed. Electrophysiological recordings were performed to evaluate synaptic function.
Results: HIV Tat exposure significantly increased the expression of ferroptosis mediators, including ferritin heavy chain-1 (FTH1) and acyl-CoA synthetase long-chain family member 4 (ACSL4), in microglia and their MEVs. Ferroptosis inhibitors suppressed these changes without affecting MEV release. Autophagy inhibition potentiated Tat-mediated MEV release and ferroptotic cargo loading, while rapamycin attenuated these effects. Tat also upregulated the ferritinophagy receptor NCOA4 in microglia, which was reversed by ferroptosis inhibition and rapamycin treatment. In neurons, Tat-MEVs induced ferroptosis, mitochondrial dysfunction, increased Fe²⁺ levels, and synaptodendritic injury, marked by reduced dendritic spine density and suppressed miniature excitatory postsynaptic currents. These effects were abrogated in neurons exposed to MEVs derived from ferroptosis-inhibited microglia.
Conclusion: These findings reveal that HIV Tat-induced dysregulated autophagy promotes ferroptosis in microglia, facilitating the release of ferroptotic cargo via MEVs and driving neuronal injury. Targeting the ferroptosis-autophagy-MEV axis may provide a potential therapeutic strategy for mitigating HIV-associated neurodegeneration.
14:30
16 May 2025
Havana

Mỹ Mahoney, Professor, Department of Pharmacology, Physiology, and Cancer Biology, Thomas Jefferson University, United States of America
Therapeutic potential of EVs in IVIG
Extracellular vesicles (EVs) play a critical factor in intercellular communication and immune regulation. Intravenous (IVIg) immunoglobulin (Ig) replacement therapy boosts the immune system in patients with antibody deficiencies or suppresses inflammation in autoimmune, infectious, and chronic inflammatory diseases. Here, we suggest a novel mechanism of immunomodulation through IVIg EV-associated cytokines. EVs were isolated from IVIg and unprocessed human plasma (UHP) from healthy donors. Bead-based multiplex analysis of known EV surface proteins by flow cytometry showed that IVIg EVs displayed significantly reduced platelet markers and enriched stemness markers compared to UHP. Luminex multiplex cytokine immune profiling revealed that the levels of 22 pro-inflammatory cytokines were dramatically reduced in IVIg EVs compared to UHP EVs. Interestingly, high levels of the pro-inflammatory cytokine IFN-γ were detected in IVIg EV-rich fractions. Here, we demonstrate that IFN-γ binds to EVs, most likely through circulating anti-IFN-γ antibodies, blocking IFN-γ-induced JAK/STAT1 activation. In summary, IVIg EVs can function as decoys to suppress inflammatory signaling pathways.
15:00
16 May 2025
Havana

Chukwumaobim Nwokwu, Assistant Professor of Biochemistry, Florida Gulf Coast University, United States of America
Liposomes as a Successful, Safe and Sustainable Drug Delivery Platform for Kaempferol in the Treatment of Glioblastoma
Conventional chemotherapeutics present problems from poor pharmacokinetic profile to toxicity to healthy tissues, which have hampered their clinical application. Nano-pharmaceutical formulations using lipid-based and polymeric materials have shown great promise in circumventing these drawbacks, because of their biocompatibility, biodegradability, targeted delivery potential, and versatile drug-loading capacity. The putative anticancer compound, kaempferol was encapsulated in liposomes (Lip-K), and its cytotoxic and apoptotic effects against human glioblastoma cells (LN-229) and healthy human astrocytes were investigated. Cytomorphological and biochemical evaluations confirmed apoptotic indices, which continued unabated even in the presence of free radical scavengers. Overall, the results suggest that liposome-encapsulated kaempferol offers an effective and relatively safe cancer treatment option, with the promise of overcoming antioxidant-induced drug resistance. Unlike most cancer therapeutics, the ability of liposomes to cross the blood-brain barrier sheds light on the potential use of liposomal kaempferol to treat glioblastoma.
15:30
16 May 2025
Coconut Grove
Mid-Afternoon Coffee Break and Networking with the Exhibitors